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    V450 Rat IgG2a, κ Isotype Control
    V450 Rat IgG2a, κ Isotype Control
    Analysis of BD Horizon™ V450 Rat IgG2a, κ Isotype Control on mouse bone marrow. The cells were stained with BD Horizon™ V450 Rat IgG2a, κ Isotype Control (solid line) or left unstimulated (dotted line). Flow cytometry was performed on a BD™ LSR II flow cytometry system.
    V450 Rat IgG2a, κ Isotype Control
    Analysis of BD Horizon™ V450 Rat IgG2a, κ Isotype Control on mouse bone marrow. The cells were stained with BD Horizon™ V450 Rat IgG2a, κ Isotype Control (solid line) or left unstimulated (dotted line). Flow cytometry was performed on a BD™ LSR II flow cytometry system.
    Product Details
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    BD Horizon™
    Rat LOU, also known as Louvain, LOU/C, LOU/M IgG2a, κ
    Mouse Pooled Immunoglobulin
    Flow cytometry, Isotype control (Routinely Tested)
    0.2 mg/ml
    AB_1645287
    Aqueous buffered solution containing protein stabilizer and ≤0.09% sodium azide.
    RUO


    Preparation And Storage

    Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon™ V450 under optimum conditions, and unreacted BD Horizon™ V450 was removed.
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    Product Notices

    1. An isotype control should be used at the same concentration as the antibody of interest.
    2. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
    3. BD Horizon V450 has a maximum absorption of 406 nm and maximum emission of 450 nm. Before staining with this reagent, please confirm that your flow cytometer is capable of exciting the fluorochrome and discriminating the resulting fluorescence.
    4. Pacific Blue™ is a trademark of Molecular Probes, Inc., Eugene, OR.
    5. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
    6. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
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    560377 Rev. 2
    Antibody Details
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    R35-95

    The R35-95 hybridoma was generated by hybridization of Y3 myeloma cells with spleen cells from LOU rats immunized with mouse immunoglobulins. The R35-95 hybridoma produces rat IgG2a, κ immunoglobulin that has no measurable reactivity with mouse immunoglobulins. The R35-95 immunoglobulin was selected as an isotype control following screening for low background binding on a variety of mouse and human tissues.

    The antibody is conjugated to BD Horizon™ V450, which has been developed for use in multicolor flow cytometry experiments and is available exclusively from BD Biosciences. It is excited by the Violet laser Ex max of 406 nm and has an Em Max at 450 nm. Conjugates with BD Horizon™ V450 can be used in place of Pacific Blue™ conjugates.

    560377 Rev. 2
    Format Details
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    V450
    BD Horizon™ V450 Dye is part of the BD Horizon™ violet family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 405-nm and an emission maximum (Em Max) at 450-nm. BD Horizon™ V450, driven by BD innovation, is designed to be excited by the violet laser (405 nm) and detected using an optical filter centered near 450-nm (e.g., a 450/50-nm bandpass filter). The dye can be excited by the UV (355-nm) laser resulting in cross-laser excitation and spillover. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
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    V450
    Violet 405 nm
    405 nm
    450 nm
    560377 Rev.2
    Citations & References
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    View product citations for antibody "560377" on CiteAb

    Development References (6)

    1. Hsu SM, Raine L, Fanger H. A comparative study of the peroxidase-antiperoxidase method and an avidin-biotin complex method for studying polypeptide hormones with radioimmunoassay antibodies. Am J Clin Pathol. 1981; 75(5):734-738. (Methodology). View Reference
    2. Hsu SM, Raine L, Fanger H. Use of avidin-biotin-peroxidase complex (ABC) in immunoperoxidase techniques: a comparison between ABC and unlabeled antibody (PAP) procedures. J Histochem Cytochem. 1981; 29(4):577-580. (Methodology). View Reference
    3. Prussin C, Metcalfe DD. Detection of intracytoplasmic cytokine using flow cytometry and directly conjugated anti-cytokine antibodies. J Immunol Methods. 1995; 188(1):117-128. (Biology). View Reference
    4. Roederer M, Kantor AB, Parks DR, Herzenberg LA. Cy7PE and Cy7APC: bright new probes for immunofluorescence. Cytometry. 1996; 24(3):191-197. (Biology). View Reference
    5. Shapiro HM. Practical Flow Cytometry, 3rd Edition. New York: Wiley-Liss, Inc; 1995:280-281.
    6. Takizawa F, Kinet JP, Adamczewski M. Binding of phycoerythrin and its conjugates to murine low affinity receptors for immunoglobulin G. J Immunol Methods. 1993; 162(2):269-272. (Biology). View Reference
    View All (6) View Less
    560377 Rev. 2

     

    Please refer to Support Documents for Quality Certificates


    Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


    Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

    For Research Use Only. Not for use in diagnostic or therapeutic procedures.