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FITC Rat IgG1 κ Isotype Control
FITC Rat IgG1 κ Isotype Control

Profile of intracellular staining of rat IgG1 isotype control (R3-34) on permeabilized, SEB+CD28/49d-activated, CD3+ peripheral blood lymphocytes of cynomolgus macaque (Macaca mulatta) monkey analyzed by flow cytometry

Profile of intracellular staining of rat IgG1 isotype control (R3-34) on permeabilized, SEB+CD28/49d-activated, CD3+ peripheral blood lymphocytes of cynomolgus macaque (Macaca mulatta) monkey analyzed by flow cytometry

Product Details
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BD Pharmingen™
Rat IgG1, κ
Mouse immunoglobulin
Flow cytometry, Intracellular staining (flow cytometry), Isotype control (Routinely Tested)
20 µl
AB_394521
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with FITC under optimum conditions, and unreacted FITC was removed. Store undiluted at 4°C.

Recommended Assay Procedures

An isotype control should be used at the same concentration as the antibody of interest.

Product Notices

  1. This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
  2. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  3. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  5. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
552916 Rev. 3
Antibody Details
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R3-34

Following immunization of a rat with mouse immunoglobulin (Ig), the Ig from the R3-34 hybridoma was identified as a non-reactive clone. The R3-34 immunoglobulin was selected as an Ig isotype control following screening for low background staining on a variety of mouse and human cells and tissues.

This reagent has been used as isotype control in immunophenotypic studies with baboon, rhesus, or cynomolgus peripheral blood cells.

This antibody is routinely tested by flow cytometric analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.

552916 Rev. 3
Format Details
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FITC
Fluorescein (FITC) is part of the BD blue family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 494-nm and an emission maximum (Em Max) at 518-nm. FITC is designed to be excited by the Blue laser (488-nm) and detected using an optical filter centered near 520 nm (e.g., a 530/30-nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
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FITC
Blue 488 nm
494 nm
518 nm
552916 Rev.3

Please refer to Support Documents for Quality Certificates


Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.