PE Mouse Anti-Human CD58

BD™ PE Mouse Anti-Human CD58

Name: PE Mouse Anti-Human CD58
Brand: BD™
SKU: 340295

Clone L306.4

(RUO (GMP))

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Product Details

Brand: BD™

Alternative Name: LFA-3; LFA3; Lymphocyte function-associated antigen 3; Ag3

Reactivity: Human

Isotype: Mouse IgG2a, κ

Immunogen: Lc-LFA-3 mouse L-cell transfectant

Application: Flow cytometry

Vol. Per Test: 20 μL

Workshop Number: V πNK15

Entrez Gene ID: 965

Storage Buffer: Phosphate buffered saline with gelatin and 0.1% sodium azide.

Regulatory Status: RUO (GMP)

Preparation And Storage

The PE conjugate is supplied in 1.0 mL of phosphate-buffered saline containing gelatin and 0.1% sodium azide. Each lot is bottled at titer as determined by immunofluorescence staining of the JY cell line. You may wish to titer the reagent for use in your specific application. Please refer to the vial label for antibody concentration. The vial should be stored at 2º to 8ºC. Conjugated forms should not be frozen and should be protected from prolonged exposure to light. Each reagent is stable for the period shown on the bottle label when stored as directed.

340295 Rev. 1

Antibody Details

L306.4

The CD58 (Anti–LFA-3) antibody, clone L306.4, is derived from the hybridization of Sp2/0 mouse myeloma cells with spleen cells isolated from C3H/HeN mice immunized with Lc–LFA-3 mouse L-cell transfectant.

The CD58 antibody (Anti–LFA-3) recognizes a 40- to 65-kilodalton (kDa) cell-surface glycoprotein that is a member of the immunoglobulin superfamily. The CD58 antigen, known as the lymphocyte function-associated antigen-3 (LFA-3), mediates cellular adhesion and participates in signal transduction when it binds to its ligand, the CD2 antigen. Cellular interactions regulated by the CD58/CD2 antigens are involved in the antigen-independent adhesion pathway and cytotoxic T lymphocyte (CTL) activity. The CD58 antigen has two isoforms. One isoform is anchored in the cell membrane by a glycophosphatidyl inositol tail, while the other has a transmembrane hydrophobic segment and a cytoplasmic segment composed of 12 amino acids.

340295 Rev. 1

Format Details

R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.

Format: PE

Excitation Source: Yellow-Green 488 nm, 532 nm, 561 nm

Excitation Max: 496 nm, 566 nm

Emission Max: 576 nm

340295 Rev.1

Citations & References

View product citations for antibody "340295" on CiteAb

Development References (12)

Rincón J, Patarroyo M. Effect of antibodies from the T-cell (‘CD2 only’) and the NK/non-lineage (new panel only) sections on adhesion of Jurkat (T) cells to human erythrocytes. In: Knapp W. W. Knapp .. et al., ed. Leucocyte typing IV : white cell differentiation antigens. Oxford New York: Oxford University Press; 1989:718-720.
Carpén O, Dustin ML, Springer TA, Swafford JA, Beckett LA, Caulfield JP. Motility and ultrastructure of large granular lymphocytes on lipid bilayers reconstituted with adhesion receptors LFA-1, ICAM-1, and two isoforms of LFA-3.. J Cell Biol. 1991; 115(3):861-71. (Biology). View Reference
Dengler TJ, Hoffmann JC, Knolle P, et al. Structural and functional epitopes of the human adhesion receptor CD58 (LFA- 3). Eur J Immunol. 1992; 22:2809-2817. (Biology).
Griffin H, Rowe M, Murray R, Brooks J, Rickinson A. Restoration of the LFA-3 adhesion pathway in Burkitt's lymphoma cells using an LFA-3 recombinant vaccinia virus: consequences for T cell recognition.. Eur J Immunol. 1992; 22(7):1741-8. (Biology). View Reference
Krensky AM, Robbins E, Springer TA, Burakoff SJ. LFA-1, LFA-2, and LFA-3 antigens are involved in CTL-target conjugation.. J Immunol. 1984; 132(5):2180-2. (Biology). View Reference
Krensky AM, Sanchez-Madrid F, Robbins E, Nagy JA, Springer TA, Burakoff SJ. The functional significance, distribution, and structure of LFA-1, LFA-2, and LFA-3: cell surface antigens associated with CTL-target interactions.. J Immunol. 1983; 131(2):611-6. (Biology). View Reference
Scheeren RA, Koopman G, Van der Baan S, Meijer CJ, Pals ST. Adhesion receptors involved in clustering of blood dendritic cells and T lymphocytes.. Eur J Immunol. 1991; 21(5):1101-5. (Biology). View Reference
Selvaraj P, Plunkett ML, Dustin M, Sanders ME, Shaw S, Springer TA. The T lymphocyte glycoprotein CD2 binds the cell surface ligand LFA-3.. Nature. 326(6111):400-3. (Biology). View Reference
Shaw S, Ginther-Luce CE, Quinones R, Gress RE, Springer TA, Sanders ME. Two antigen-independent adhesion pathways used by human cytotoxic T-cell clones. Nature. 1989; 714-716. (Biology).
Shaw S, Ginther-Luce CE, Quinones R, Gress RE, Springer TA, Sanders ME. Two antigen-independent adhesion pathways used by human cytotoxic T-cell clones. Nature. 1989; 714-716. (Biology).
Smith ME, Thomas JA. Cellular expression of lymphocyte function associated antigens and the intercellular adhesion molecule-1 in normal tissue.. J Clin Pathol. 1990; 43(11):893-900. (Biology). View Reference
Springer TA. Adhesion receptors of the immune system. Nature. 1990; 346(6283):425-434. (Biology). View Reference

View All (12)

340295 Rev. 1

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