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Anti-Human CD56 FITC/CD69 PE/CD45 PerCP
Product Details
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BD FastImmune™
Human
Flow cytometry
RUO (GMP)
Phosphate buffered saline with BSA and 0.1% sodium azide.


Description

The CD56 antibody, clone NCAM16.2, is derived from the hybridization of P3X-63- Ag8.653 mouse cells with lymph nodes from BALB/c mice immunized with immunoaffinity-enriched adult human brain NCAM.

The CD69 antibody, clone L78, is derived from hybridization of Sp2/0-Ag14 mouse myeloma cells with lymph node cells from BALB/c mice immunized with a CD8+ alloantigen-directed CTL cell line.

The CD45 antibody, clone 2D1, is derived from hybridization of NS-1 mouse myeloma cells with spleen cells from BALB/c mice immunized with human peripheral blood mononuclear cells (PBMCs).

The CD56 (NCAM16.2) antibody recognizes an extracellular immunoglobulin-like domain common to three molecular weight forms—120, 140, and 180 kilodaltons (kDa)—of the neural cell adhesion molecule (NCAM).

The CD69 antibody recognizes a very early human lymphocyte activation antigen. The CD69 antigen is a surface homodimer formed by the association of 28-kDa and 32-kDa chains that are held together by disulfide bridges.

The CD45 antibody recognizes a 180- to 220-kDa human leucocyte antigen that is a member of the leucocyte common antigen (LCA) family.


The CD56 antibody, clone NCAM16.2, is derived from the hybridization of P3X-63- Ag8.653 mouse cells with lymph nodes from BALB/c mice immunized with immunoaffinity-enriched adult human brain NCAM.

The CD69 antibody, clone L78, is derived from hybridization of Sp2/0-Ag14 mouse myeloma cells with lymph node cells from BALB/c mice immunized with a CD8+ alloantigen-directed CTL cell line.

The CD45 antibody, clone 2D1, is derived from hybridization of NS-1 mouse myeloma cells with spleen cells from BALB/c mice immunized with human peripheral blood mononuclear cells (PBMCs).

The CD56 (NCAM16.2) antibody recognizes an extracellular immunoglobulin-like domain common to three molecular weight forms—120, 140, and 180 kilodaltons (kDa)—of the neural cell adhesion molecule (NCAM).

The CD69 antibody recognizes a very early human lymphocyte activation antigen. The CD69 antigen is a surface homodimer formed by the association of 28-kDa and 32-kDa chains that are held together by disulfide bridges.

The CD45 antibody recognizes a 180- to 220-kDa human leucocyte antigen that is a member of the leucocyte common antigen (LCA) family.

Preparation And Storage

Store vials at 2°C–8°C. Conjugated forms should not be frozen. Protect from exposure to light. Each reagent is stable until the expiration date shown on the bottle label when stored as directed.

340417 Rev. 1
Components
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Description Clone Isotype
CD45 PerCP C8Mab-1 IgG2b, λ
CD69 PE 2D7/Basophils IgG1, κ
CD56 FITC NCAM16.2 IgG2b, κ
340417 Rev. 1
Citations & References
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Development References (21)

  1. Centers for Disease Control. Update: universal precautions for prevention of transmission of human immunodeficiency virus, hepatitis B virus, and other bloodborne pathogens in healthcare settings. MMWR. 1988; 37:377-388. (Biology).
  2. Chen JH, Prince H, Buck D, et al. Leu-23: an early activation antigen on human lymphocytes. Fed Proc. 1988; 2:A1214. (Biology).
  3. Clinical and Laboratory Standards Institute. 2005. (Biology).
  4. Cobbold SP, Hale G, Waldmann H. Non-lineage, LFA-1 family, and leucocyte common antigens: new and previously defined clusters. In: McMichael AJ. A.J. McMichael .. et al., ed. Leucocyte typing III : white cell differentiation antigens. Oxford New York: Oxford University Press; 1987:788-803.
  5. Cunningham BA, Hemperly JJ, Murray BA, Prediger EA, Brackenbury R, Edelman GM. Neural cell adhesion molecule: structure, immunoglobulin-like domains, cell surface modulation, and alternative RNA splicing. Science. 1987; 236(4803):799-806. (Biology). View Reference
  6. Edelman GM. Cell adhesion molecules in the regulation of animal form and tissue pattern. Annu Rev Cell Biol. 1986; 2:81-116. (Biology). View Reference
  7. Lanier LL, Buck DW, Rhodes L, et al. Interleukin 2 activation of natural killer cells rapidly induces the expression and phosphorylation of the Leu-23 activation antigen.. J Exp Med. 1988; 167(5):1572-85. (Biology). View Reference
  8. Lanier LL, Chang C, Azuma M, Ruitenberg JJ, Hemperly JJ, Phillips JH. Molecular and functional analysis of human natural killer cell-associated neural cell adhesion molecule (N-CAM/CD56). J Immunol. 1991; 146(12):4421-4426. (Biology). View Reference
  9. Lanier LL, Le AM, Civin CI, Loken MR, Phillips JH. The relationship of CD16 (Leu-11) and Leu-19 (NKH-1) antigen expression on human peripheral blood NK cells and cytotoxic T lymphocytes. J Immunol. 1986; 136(12):4480-4486. (Biology). View Reference
  10. Lanier LL, Testi R, Bindl J, Phillips JH. Identity of Leu-19 (CD56) leukocyte differentiation antigen and neural cell adhesion molecule. J Exp Med. 1989; 169(6):2233-2238. (Biology). View Reference
  11. Ledbetter JA, Evans RL, Lipinski M, Cunningham-Rundles C, Good RA, Herzenberg LA. Evolutionary conservation of surface molecules that distinguish T lymphocyte helper/inducer and cytotoxic/suppressor subpopulations in mouse and man. J Exp Med. 1981; 153(2):310-323. (Biology). View Reference
  12. Maino VC, Suni MA, Ruitenberg JJ. Rapid flow cytometric method for measuring lymphocyte subset activation.. Cytometry. 1995; 20(2):127-33. (Biology). View Reference
  13. Phillips JH, Lanier LL. Dissection of the lymphokine-activated killer phenomenon: relative contribution of peripheral blood natural killer cells and T lymphocytes to cytolysis. J Exp Med. 1986; 164(3):814-825. (Biology). View Reference
  14. Schubert J, Lanier LL, Schmidt RE. Knapp W, Dörken B, Gilks WR, et al, ed. Leucocyte Typing IV: White Cell Differentiation Antigens. New York, NY: Oxford University Press; 1989:699-702.
  15. Schwarting R, Biedobitek G, Stein H. Knapp W, Dörken B, Gilks WR, et al, ed. Leucocyte Typing IV: White Cell Differentiation Antigens. New York, NY: Oxford University Press; 1989:428-432.
  16. Schwinzer R. Cluster Report: CD45/CD45R. In: Knapp W. W. Knapp .. et al., ed. Leucocyte typing IV : white cell differentiation antigens. Oxford New York: Oxford University Press; 1989:628-634.
  17. Testi R, D'Ambrosio D, De Maria R, Santoni A. The CD69 receptor: a multipurpose cell-surface trigger for hematopoietic cells. Immunol Today. 1994; 15:479-483. (Biology).
  18. Testi R, Philips J, Lanier LL. Constitutive expression of a phosphorylated activation (Leu-23) by CD3 bright thymocytes. J Immunol. 1988; 141:2257. (Biology).
  19. Testi R, Philips JH, Lanier LL. Leu-23 induction as an early marker for functional CD3/T cell antigen receptor triggering: requirement for receptor cross-linking, prolonged elevation of intracellular (Ca ++ ), and stimulation of protein kinase C. J Immunol. 1988; 2:1214. (Biology).
  20. Testi R, Phillips JH, Lanier LL. T cell activation via Leu-23 (CD69).. J Immunol. 1989; 143(4):1123-8. (Biology). View Reference
  21. Testi R, Pulcinelli F, Frati L, Gazzaniga PP, Santoni A. CD69 is expressed on platelets and mediates platelet activation and aggregation.. J Exp Med. 1990; 172(3):701-7. (Biology). View Reference
View All (21) View Less
340417 Rev. 1

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