γ2a (IgG2a), clone X39, and γ1 (IgG1), clone X40, are both derived from hybridization of mouse Sp2/0-Ag14 myeloma cells with spleen cells from BALB/c mice immunized with KLH.
CD4, clone SK3, is derived from hybridization of mouse NS-1 myeloma cells with spleen cells from BALB/c mice immununized with human peripheral blood T lymphocytes.
CD3, clone SK7, is derived from hybridization of mouse NS-1 myeloma cells with spleen cells from BALB/c mice immunized with human thymocytes.
Both γ2a (IgG2a) and γ1 (IgG1) react speciﬁcally with keyhole limpet hemocyanin (KLH), an antigen not expressed on human cells or human cell lines.
The CD4 antibody recognizes an antigen, with a molecular weight of 55-kilodalton (kDa) that is present on T-helper/inducer lymphocytes and monocytes.
CD3 reacts with the epsilon chain of the CD3 antigen/T-cell antigen receptor (TCR) complex. This complex is composed of at least six proteins that range in molecular weight from 20 to 30 kd. The antigen recognized by CD3 antibodies is noncovalently associated with either α/β or γ/δ TCR (70 to 90 kd).