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    CD64 BV605

    BD Horizon™ CD64 BV605

    Clone 10.1

    (IVD)
    Product Details
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    Description

    The CD64 antibody, clone 10.1, is derived from the hybridization of Sp2/0-Ag14 mouse myeloma cells with spleen cells isolated from a BALB/c mouse that was immunized first with 2 x 107 rheumatoid synovial fluid cells and on subsequent occasions with 1.5 x 107 fibronectin-purified human monocytes obtained from pools of blood group–matched donors.

    CD64 recognizes the 72-kilodalton (kDa) human FcγRI that can bind monomeric IgG.



    Preparation And Storage

    The antibody reagent is stable until the expiration date shown on the label when stored at 2° to 8°C. Do not use after the expiration date. Do not freeze the reagent or expose it to direct light during storage or incubation with cells. Keep the reagent vial dry. Do not use the reagent if you observe any change in appearance. Precipitation or discoloration indicates instability or deterioration. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions and unconjugated antibody and free dye were removed.
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    Product Notices

    1. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
    2. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
    3. For U.S. patents that may apply, see bd.com/patents.
    4. This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
    5. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
    6. An isotype control should be used at the same concentration as the antibody of interest.
    7. Although every effort is made to minimize the lot-to-lot variation in the efficiency of the fluorochrome energy transfer, differences in the residual emission from BD Horizon™ BV421 may be observed. Therefore, we recommend that individual compensation controls be performed for every BD Horizon™ BV605 conjugate.
    8. Please observe the following precautions: We recommend that special precautions be taken (such as wrapping vials, tubes, or racks in aluminum foil) to protect exposure of conjugated reagents, including cells stained with those reagents, to any room illumination. Absorption of visible light can significantly affect the emission spectra and quantum yield of tandem fluorochrome conjugates.
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    666673 Rev. 1

    Please refer to Support Documents for Quality Certificates

    Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

    Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

    For In Vitro Diagnostics Use.

    Documents are subject to revision without notice. Please verify you have the correct revision of the document, and always refer back to BD's eIFU website for the latest and most up to date information.