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Anti-Cytokeratin Purified
Product Details
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BD™
Cytokeratin-7/-8; CK-7/CK-8;
Human
Mouse BALB/c IgG2a
Human HT29 colorectal carcinoma cell line
Immunohistochemistry
25 μg/mL
20 μL
AB_2800363
Phosphate buffered saline with gelatin and 0.1% sodium azide.
CE_IVD


Preparation And Storage

The antibody reagent is stable until the expiration date shown on the label when stored at 2° to 8°C. Do not use after the expiration date. Do not freeze the reagent or expose it to direct light during storage or incubation with cells. Keep the outside of the reagent vial dry.

Do not use the reagent if you observe any change in appearance. Precipitation or discoloration indicates instability or deterioration.

345779 Rev. 1
Antibody Details
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CAM5.2

The Anti-Cytokeratin (CAM 5.2) reagent is a murine monoclonal antibody used for qualitative identification of normal cells and malignant cells of epithelial origin in tissue sections (paraffin and frozen) and cytocentrifuge preparations. The clinical interpretation of any staining or its absence should be complemented by morphological studies and proper controls (such as positive, negative, and isotype-matched controls). All tests should be performed by a qualified individual.

345779 Rev. 1
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
345779 Rev.1
Citations & References
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Development References (16)

  1. A National Committee for Clinical Laboratory Standards. Procedures for the Collection of Diagnostic Blood Specimens by Venipuncture (H3-A3). 1991. (Biology).
  2. Battifora H. Diagnostic uses of antibodies to keratins: A review and immunohistochemical comparison of seven monoclonal and three polyclonal antibodies. Prog Surg Pathol. 1990; 8:1-15. (Biology).
  3. Cooper D, Schermer A, Sun TT. Biology of disease classification of human epithelia and their neoplasms using monoclonal antibodies to keratins: strategies, applications and limitations. Lab Invest. 1985; 52:243-256. (Biology). View Reference
  4. Gatter K, Ralfkiaer E, Skinner J, et al. An immunochemical study of malignant melanoma and its differential diagnosis from other malignant tumors. J Clin Path. 1985; 38:1353-1357. (Biology).
  5. Hofman, F. Immunoperoxidase staining of cell surfaces: cytocentrifuge preparations. Becton Dickinson Monoclonal Antibodies Source Book. 1989; 1-8. (Biology).
  6. Johnson D, Warnke R, Herndier B, Rouse R. An immunohistochemical study of the cytokeratin profiles of hepatocellular carcinomas and cholangoicarcinomas. Lab Investig. 1987; 56:34A. (Biology).
  7. Leader M, Patel J, Makin C, Henry K. An analysis of the sensitivity and specificity of the cytokeratin marker CAM 5.2 for epithelial tumors: results of a study of 203 sarcomas, 50 carcinomas, and 28 malignant melanomas. Histopathology. 1986; 10:1315-1324. (Biology). View Reference
  8. Makin C, Bobrow L, Bodmer W. Monoclonal antibody to cytokeratin for use in routine histopathology. J Clin Pathol. 1984; 37(9):975-983. (Biology). View Reference
  9. Moll R, Franke W, Schiller D, Geiger B, Krepler R. The catalog of human cytokeratins: patterns of expression in normal epithelia, tumors, and cultured cells. Cell. 1982; 31:11-24. (Biology). View Reference
  10. National Committee for Clinical Laboratory Standards. Protection of Laboratory Workers from Infectious Disease Transmitted by Blood, Body Fluids, and Tissue: Tentative Guideline (M29-T2). 1991. (Biology).
  11. Pitts M, Rojas B, Rouse R, Kempson R. An immunohistochemical study of metaplastic carcinomas and sarcomas of the breast. Lab Investig. 1987; 56:61A. (Biology).
  12. Robbins SL, Cotran RS, Kumar V. Pathologic Basis of Disease. 1984. (Biology).
  13. Rose NR, et al. Manual of Clinical Laboratory Immunology 3rd ed. Washington, DC: American Society for Microbiology; 1986:234.
  14. Smedts F, Ramaekers F, Robben H, et al. Changing patterns of keratin expression during progression of cervical intraepithelial neoplasia. Am J Pathol. 1990; 136(3):657-668. (Biology). View Reference
  15. Theaker J, Gatter K, Esiri M, et al. Epithelial membrane antigen and cytokeratin expression by meningiomas: an immunohistochemical study. J Clin Pathol. 1986; 39:435-439. (Biology).
  16. Wood G, Warnke R. Suppression of endogenous avidin-binding activity in tissues and its relevance to biotin-avidin detection systems. J Histochem Cytochem. 1981; 29:1196-1204. (Biology).
View All (16) View Less
345779 Rev. 1

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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For In Vitro Diagnostic Use.

 

23-22942-00