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γ1 FITC/γ1 PE/CD45 PerCP
Product Details
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BD Tritest™
Human
Controls
CE_IVD
Phosphate buffered saline with BSA and 0.1% sodium azide.


Description

BD Tritest™ Control, γfluorescein isothiocyanate (FITC)/γphycoerythrin (PE)/CD45 peridinin chlorophyll (PerCP), is a three-color direct immunofluorescence reagent used as an isotype (negative) control for flow cytometric immunophenotyping of erythrocyte-lysed whole blood with Tritest reagents on the Becton Dickinson family of flow cytometers. The Tritest Control is used to determine the unstained lymphocyte population on a fluorescence 1 (FL1) vs fluorescence 2 (FL2) display and to determine any nonantigen-specific antibody binding (nonspecific staining) present, particularly that caused by Fc receptors. BDIS Tritest Control is for in vitro diagnostic use with BD in vitro diagnostic Tritest reagents.

Preparation And Storage

1. For in vitro diagnostic use.

2. Store the reagent at 2° to 8°C to maintain stability. Do not use after expiration date shown on the label.

3. Do not freeze the reagent or expose it to direct light during storage or during incubation with cells. Keep the reagent vial dry.

4. Do not use the reagent if you observe any changes in appearance. Precipitation or discoloration indicates instability or deterioration.

5. The antibody reagent contains sodium azide as a preservative; however, care should be taken to avoid microbial contamination, which may cause erroneous results.

342415 Rev. 1
Components
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Description Clone Isotype EntrezGene ID
CD45 PerCP 2D1 IgG1, κ N/A
Mouse IgG1 Isotype Control PE L293 IgG1, κ N/A
Mouse IgG1 Isotype Control FITC X40 IgG1, κ N/A
342415 Rev. 1
Citations & References
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Development References (11)

  1. A National Committee for Clinical Laboratory Standards. Procedures for the Collection of Diagnostic Blood Specimens by Venipuncture (H3-A3). 1991. (Biology).
  2. Centers for Disease Control. Revised Guidelines for the Performance of CD4+ T-Cell Determinations in Persons with HIV Infection. MMWR. 1994; 43:1-21. (Biology).
  3. Centers for Disease Control. Update: universal precautions for prevention of transmission of humanimmunodeficiency virus, hepatitis B virus, and other bloodborne pathogens in health-care settings. MMWR. 1988; 37:377-388. (Biology).
  4. Cobbold SP, Hale G, Waldmann H. Non-lineage, LFA-1 family, and leucocyte common antigens: new and previously defined clusters. In: McMichael AJ. A.J. McMichael .. et al., ed. Leucocyte typing III : white cell differentiation antigens. Oxford New York: Oxford University Press; 1987:788-803.
  5. Giorgi JV. Lymphocyte subset measurements: significance in clinical medicine. In: Rose NR, Friedman H, Fahey JL, ed. Manual of Clinical Laboratory Immunology. 3rd ed.. Washington, DC: American Society for Microbiology; 1986:236-246.
  6. Jackson AL, Warner NL. Rose NR, Friedman H, Fahey JL, ed. Manual of Clincial Laboratory Immunology, Third Edition. Washington DC: American Society for Microbiology; 1986:226-235.
  7. National Committee for Clinical Laboratory Standards. Clinical Applications of Flow Cytometry: Quality Assurance and Immunophenotyping of Peripheral Blood Lymphocytes; Tentative Guideline. 1992; 1992. (Biology).
  8. National Committee for Clinical Laboratory Standards. Protection of Laboratory Workers from Infectious Disease Transmitted by Blood, Body Fluids, and Tissue: Tentative Guideline (M29-T2). 1991. (Biology).
  9. Nicholson J, Browning S, Orloff S, McDougal J. Inactivation of HIV-infected H9 cells in whole blood preparations by lysing/fixing reagents used in flow cytometry. J Immunol Methods. 1993; 160:215-218. (Biology).
  10. Nicholson J, Jones B, Hubbard M. CD4 Tlymphocyte determinations on whole blood specimens using a single-tube three-color assay. Cytometry. 1993; 14:598-605. (Biology).
  11. Schwinzer R. Cluster Report: CD45/CD45R. In: Knapp W. W. Knapp .. et al., ed. Leucocyte typing IV : white cell differentiation antigens. Oxford New York: Oxford University Press; 1989:628-634.
View All (11) View Less
342415 Rev. 1

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For In Vitro Diagnostic Use.

 

23-22942-00