Reagent Solutions
During this challenging time, researchers at the forefront of COVID-19 research need tools to power deeper cellular analysis. BD Biosciences research reagents include innovative dyes and a broad portfolio of human-specific antibodies to deliver more resolution and flexibility for experimental design, saving valuable samples and accelerating the time to critical insights.
Optimized Panels, Kits and Assays
Readily available optimized antibody cocktails, gene panels, kits and recommended multicolor flow cytometry panels shown in the tables below can help expedite experiments relevant to COVID-19 research.
*Please contact your sales representative to request a quote.
| Research Area |
Research Application |
Recommended Research Panel |
| Viral Immune Response |
Assess the expression of multiple T cell inhibitory receptors (PD-1, TIM-3, LAG-3, CTLA-4, BTLA, TIGIT) on naïve and different memory T cell subsets |
T Cell Exhaustion Panel |
| Marker |
Dye |
Clone |
Cat. No |
| CD3 |
PerCP-Cy5.5 |
UCHT1 |
560835 |
| CD4 |
APC-R700 |
RPA-T4 |
564975 |
| CD8 |
APC-H7 |
SK1 |
560179 |
| CD45RA |
BV650 |
HI100 |
563963 |
| CD197 (CCR7) |
FITC |
150503 |
561271 |
| CD95 |
BV480 |
DX2 |
746675 |
| TIGIT |
BV421 |
741182 |
747844 |
| CD152 (CTLA-4) |
PE |
BNI3 |
555853 |
| CD223 (LAG-3) |
Alexa Fluor® 647 |
T47-530 |
565716 |
| CD272 (BTLA) |
BV605 |
J168-540 |
743986 |
| CD279 (PD-1) |
PE-Cy7 |
EH12.1 |
561272 |
| CD366 (TIM-3) |
BV786 |
7D3 |
742857 |
| Viral Immune Response |
Identification of T helper cell (Th) subsets (Th1, Th2, Th17, Th22), follicular T helper cell (Tfh) subsets, regulatory T cell (Treg) subsets, and follicular regulatory T cell (Tfr) subsets |
T Cell Subset Panel |
| Marker |
Dye |
Clone |
Cat. No |
| Live/Dead |
7-AAD |
N/A |
559925 |
| CD3 |
Alexa Fluor® 700 |
UCHT1 |
557943 |
| CD4 |
APC-H7 |
SK3 |
641398 |
| CD45RA |
BV605 |
HI100 |
562886 |
| CD127 |
BV711 |
HIL-7R-M21 |
563165 |
| CD25 |
BB515 |
2A3 |
564467 |
| CD183 (CXCR3) |
PE-Cy7 |
1C6/CXCR3 |
560831 |
| CD185 (CXCR5) |
BV480 |
RF8B2 |
566142 |
| CD194 (CCR4) |
BV421 |
1G1 |
562579 |
| CD196 (CCR6) |
BV786 |
11A9 |
563704 |
| CD294 (CRTH2) |
PE |
BM16 |
563665 |
| CCR10 |
APC |
1B5 |
564771 |
BD Biosciences also offers a large and diverse portfolio of high-quality reagents to meet your specific experimental needs and enables the optimization and design of your own assay. Explore the availability of additional reagents.
Immunophenotyping and Cell Sorting
The immune response to a virus such as SARS-CoV-2 can be tracked in the blood. Multicolor flow cytometry is one of the most powerful tools with which to identify, for example, alterations in the immune cell landscape in response to viral infection. BD Biosciences has a broad catalog of flow cytometry reagents to aid in the analysis and isolation of cells of the innate and adaptive immune system.
For example, T cells are a key component of viral immunity, not only indirectly fighting viral infections, but also in helping to mount a successful antibody response to provide lasting immunity. The table below shows a representative list of high-quality, flow cytometry–tested antibodies available in a broad range of colors that enable a comprehensive study of murine and human T cell biology.
BD Horizon™ Dri Chroma Panels
BD offers contract manufacturing of pre-aliquoted, performance-optimized multicolor panels for flow cytometry in a dried down, ready to use format. The dried multicolor panels reduce inter-experiment variability and come with long shelf lives making them ideal choices for multisite and longitudinal studies.
Learn More
Cytokine Response
Proper cytokine response is important for the clearance of any virus. In the case of COVID-19, early research suggests that overactive responses leading to cytokine release syndrome may contribute to more severe presentation of the disease1, 2. Some cytokines that have been identified as abnormally high in COVID-19 samples include:
BD Biosciences offers different solutions for comprehensive measurement of cytokine production, either at the single-cell (intracellular flow cytometry, BD FastImmune™ Cytokine Detection System) or bulk level (BD® Cytometric Bead Array, ELISA) for COVID-19 research.
BD FastImmune™ Cytokine Detection System
With streamlined and highly reproducible intracellular cytokine staining, the BD FastImmune System is designed to meet the needs of applied research on human samples. As a complete and optimized system, it is particularly well suited for research studies assessing immune responses to virus or vaccine candidates.
BD Biosciences provides a range of BD FastImmune Multicolor or Single-Color Cytokine Reagents, produced under RUO (GMP) conditions, for antibody staining, including selected sample-activation and sample-processing reagents.
Available Cocktails:
Selected list of relevant single-color BD FastImmune Reagents for analysis of cytokine and degranulation marker expression.
| Target |
APC |
FITC |
PE |
Activating Reagents |
| CD107a |
|
|
|
|
| CD2/CD2R |
|
|
|
|
| CD28/CD49d Costimulatory Reagent |
|
|
|
|
| IFN-γ |
|
|
|
|
| IL-13 |
|
|
|
|
| IL-1ra |
|
|
|
|
| IL-1α |
|
|
|
|
| IL-1β |
|
|
|
|
| IL-2 |
|
|
|
|
| IL-4 |
|
|
|
|
| IL-6 |
|
|
|
|
| IL-8 |
|
|
|
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| TNF |
|
|
|
|
BD® Cytometric Bead Array (CBA)
BD® CBA solutions are available in preconfigured kits or in an open, configurable format for unique needs.
BD CBA Flex Sets provide an open and configurable method of detection so that researchers can build their own multiplexes for protein quantitation as low as 10 pg/mL.
BD CBA Enhanced Sensitivity Flex Sets are capable of detecting cytokine concentrations as low as 0.274 pg/mL.
BD CBA Kits are preconfigured for achieving consistent results for routine panels.
Selected list of relevant BD CBA Flex Sets:
ELISAs
BD Biosciences offers three convenient ELISA reagent formats for quantitation of cytokines and other soluble proteins.
Individual reagents are comprised of unlabeled capture antibodies, biotinylated detection antibodies and protein standards offering a wide range of specificities and assay design flexibility. BD OptEIA™ Sets include reagents for five or twenty 96-well plates and pre-coated 96-well plates. All three BD Biosciences sandwich ELISA formats provide fast, easy quantitation of soluble analytes and total response analysis.
Selected list of relevant BD OptEIA™ ELISA Sets:
BD® AbSeq Antibodies
Utilize the cutting-edge antibody-oligo technology in the form of BD AbSeq Antibodies to probe up to 100 different cell surface proteins at a single-cell level, while simultaneously performing single-cell RNA analyses. Multiomic research using BD AbSeq Antibodies enables deeper characterization of your immune populations of interest and the potential discovery of novel cell immune signatures.
References
- Shi Y, Wang Y, Shao C, et al. COVID-19 infection: the perspectives on immune responses. Cell Death Differ. 2020; Epub ahead of print. doi: 10.1038/s41418-020-0530-3.
- Cossarizza A, De Biasi S, Guaraldi G, Girardis M, Mussini C. SARS-CoV-2, the virus that causes COVID-19: cytometry and the new challenge for global health. Cytometry A. 2020;97(4):340–343. doi: 10.1002/cyto.a.24002.