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Purified Mouse Anti-PTP1B
Product Details
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BD Transduction Laboratories™
Human (QC Testing), Mouse, Rat, Dog, Chicken, Frog (Tested in Development)
Mouse IgG2a
Human PTP1B aa. 269-435
Western blot (Routinely Tested), Immunofluorescence (Tested During Development), Immunohistochemistry, Immunoprecipitation (Not Recommended)
50 kDa
250 µg/ml
AB_397543
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
610140 Rev. 1
Antibody Details
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15/PTP1B

PTP1B is a member of the class of enzymes termed protein tyrosine phosphatases  (PTPs). This 50 kDa protein contains a conserved phosphatases domain at residues 30-278 and is localized to the cytoplasmic face of the endoplasmic reticulum by its C-terminal 35 residues. Its interactions with other proteins are mediated by proline-rich regions and SH2 compatible sequences. PTP1B is thought to act as a negative regulator in insulin signaling. Insulin stimulation induces its association with the insulin receptor and tyrosine phosphorylation. Its overexpression inhibits proximal and distal insulin signaling events, possibly due to receptor dephosphorylation. Microinjection of PTP1B into Xenopus oocytes blocks insulin-induced maturation. Additional studies demonstrate that PTP1B undergoes cell cycle-dependent and/or stress-induced serine phosphorylation. This suggests further regulatory roles that are not yet defined.

610140 Rev. 1
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
610140 Rev.1
Citations & References
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Development References (5)

  1. Bharadwaj S, Ali A, Ovsenek N. Multiple components of the HSP90 chaperone complex function in regulation of heat shock factor 1 In vivo. Mol Biol Cell. 1999; 19(12):8033-8041. (Biology). View Reference
  2. Gaits F, Li RY, Bigay J, Ragab A, Ragab-Thomas MF, Chap H. G-protein beta gamma subunits mediate specific phosphorylation of the protein-tyrosine phosphatase SH-PTP1 induced by lysophosphatidic acid. J Biol Chem. 1996; 271(33):20151-20155. (Biology). View Reference
  3. Rutledge TW, Whiteheart SW. SNAP-23 is a target for calpain cleavage in activated platelets. J Biol Chem. 2002; 277(40):37009-37015. (Clone-specific: Western blot). View Reference
  4. Tao J, Malbon CC, Wang HY. Galpha(i2) enhances insulin signaling via suppression of protein-tyrosine phosphatase 1B. J Biol Chem. 2001; 276(43):39705-39712. (Clone-specific: Immunoprecipitation, Western blot). View Reference
  5. Xu G, Arregui C, Lilien J, Balsamo J. PTP1B modulates the association of beta-catenin with N-cadherin through binding to an adjacent and partially overlapping target site. J Biol Chem. 2002; 277(51):49989-49997. (Clone-specific: ELISA, Western blot). View Reference
View All (5) View Less
610140 Rev. 1

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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

Non-IVD products are For Research Use Only. Not for use in diagnostic or therapeutic procedures.

 

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