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Purified Mouse Anti-MEK2
Product Details
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BD Transduction Laboratories™
MAP Kinase Kinase 2; ERK Kinase
Mouse (QC Testing), Human, Rat, Dog, Chicken, Frog (Tested in Development)
Mouse IgG2a
Rat MEK2 aa. 1-110 Recombinant Protein
Western blot (Routinely Tested), Immunofluorescence, Immunoprecipitation (Tested During Development), Immunohistochemistry-formalin (antigen retrieval required) (Not Recommended)
46 kDa
250 µg/ml
AB_397631
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
610235 Rev. 1
Antibody Details
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96/MEK2

MEK (Map/Erk Kinase) 1 and 2 are serine/threonine kinases, also known as MAP kinase kinases (MAP2K1 and 2, MAPKK1 and 2, or MKK1 and 2).  They activate the MAP (Mitogen-Activated Protein) kinases, also known as ERKs (Extracellular signal Regulated Kinases), which are critical kinases in multiple signal transduction pathways that regulate cell growth and differentiation.  Activation of MEK 1 and 2 is dependent upon phosphorylation of serines 218 and/or 222 by activated MAP kinase kinase kinases (MAP3Ks), such as the Raf isoforms.  Hormones, growth and differentiating factors, or tumor promoters induce Raf activation via activation of Ras proteins.  MEK2 is seven amino acids larger and shares 81% identity with MEK1. In cultured cells, MEK2 is activated by serum. In vitro, v-Raf phosphorylates and activates  MEK2. It is thought that all of these activated protein kinases are downstream of the Ras signal transduction pathway and represent an integral part of the Ras mitogenic signal.

The 96/MEK2 monoclonal antibody recognizes MEK2, regardless of phosphorylation status.

This antibody is routinely tested by western blot analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.

610235 Rev. 1
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
610235 Rev.1
Citations & References
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Development References (5)

  1. Crews CM, Alessandrini A, Erikson RL. The primary structure of MEK, a protein kinase that phosphorylates the ERK gene product. Science. 1992; 258(5081):478-480. (Biology). View Reference
  2. Downey GP, Butler JR, Tapper H, et al. Importance of MEK in neutrophil microbicidal responsiveness. J Immunol. 1998; 160(1):434-443. (Biology: Immunoprecipitation). View Reference
  3. Hattori S, Fukuda M, Yamashita T, Nakamura S, Gotoh Y, Nishida E. Activation of mitogen-activated protein kinase and its activator by ras in intact cells and in a cell-free system. J Biol Chem. 1992; 267(28):20346-20351. (Biology). View Reference
  4. Tworkowski KA, Salghetti SE, Tansey WP. Stable and unstable pools of Myc protein exist in human cells. Oncogene. 2002; 21(55):8515-8520. (Biology: Western blot). View Reference
  5. Wu J, Harrison JK, Dent P, Lynch KR, Weber MJ, Sturgill TW. Identification and characterization of a new mammalian mitogen-activated protein kinase kinase, MKK2. Mol Cell Biol. 1993; 13(8):4539-4548. (Biology). View Reference
View All (5) View Less
610235 Rev. 1

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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

Non-IVD products are For Research Use Only. Not for use in diagnostic or therapeutic procedures.

 

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