Purified Mouse Anti-ERK1

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BD Transduction Laboratories™ Purified Mouse Anti-ERK1

Name: Purified Mouse Anti-ERK1
Brand: BD Transduction Laboratories™
SKU: 610031

Clone MK12

(RUO)

Immunohistochemical staining of ERK1 in pyrimidal cells from the rat cortex: formalin-fixed paraffin-embedded tissue, with citrate pre-treatment (40X magnification).

Immunofluorescence staining of human fibroblasts.

Western blot analysis of ERK1 on a rat cerebrum lysate. Lane 1: 1:4000, lane 2: 1:8000, lane 3: 1:16,000 dilution of the mouse anti-ERK1 antibody. ERK1 is expected to appear at 44 kD with crossreactivity reported to ERK2 which may appear at 42 kD.

Immunohistochemical staining of ERK1 in pyrimidal cells from the rat cortex: formalin-fixed paraffin-embedded tissue, with citrate pre-treatment (40X magnification).

Immunofluorescence staining of human fibroblasts.

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Product Details

Brand: BD Transduction Laboratories™

Reactivity: Rat (QC Testing), Human, Mouse, Dog, Chicken, Frog (Tested in Development)

Isotype: Mouse IgG1

Immunogen: Rat ERK1 aa. 325-345

Application: Western blot (Routinely Tested), Immunofluorescence, Immunohistochemistry, Immunoprecipitation (Tested During Development)

Target Molecular Weight: 44 kDa

Concentration: 250 µg/ml

RRID: AB_397447

Storage Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.

Regulatory Status: RUO

Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.

Recommended Assay Procedures

Western blot: Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml

Product Notices

Since applications vary, each investigator should titrate the reagent to obtain optimal results.
Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Companion Products

Rat Cerebrum Lysate RUO

Size 500 µg Cat No. 611463

HRP Goat Anti-Mouse Ig RUO

Size 1 mL Cat No. 554002

FITC Goat Anti-Mouse Ig RUO

Size 0.5 mg Cat No. 554001

610031 Rev. 1

Antibody Details

MK12

The family of serine/threonine kinases known as ERKs (extracellular signal regulated kinases) or MAPKs (mitogen-activated protein kinases) is activated after cell stimulation by a variety of hormones and growth factors. Cell stimulation induces a signaling cascade that leads to phosphorylation of MEK (MAPK/ERK kinase) which, in turn, activates ERK via tyrosine and threonine phosphorylation. A myriad of proteins represent the downstream effectors for the active ERK and implicate it in the control of cell proliferation and differentiation, as well as regulation of the cytoskeleton. Activation of ERK is normally transient and cells possess dual specificity phosphatases that are responsible for its down-regulation. Furthermore, multiple studies have shown that elevated ERK activity is associated with some cancers. ERK1 is a 44 kDa member of the ERK family and shares 85% homology with ERK2 (42 kDa).

610031 Rev. 1

Format Details

Purified

Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.

Format: Purified

610031 Rev.1

Citations & References

Development References (5)

Ackerley S, Grierson AJ, Brownlees J, et al. Glutamate slows axonal transport of neurofilaments in transfected neurons. J Cell Biol. 2000; 150(1):165-175. (Biology: Western blot). View Reference
Aguirre-Ghiso JA, Liu D, Mignatti A, Kovalski K, Ossowski L. Urokinase receptor and fibronectin regulate the ERK(MAPK) to p38(MAPK) activity ratios that determine carcinoma cell proliferation or dormancy in vivo. Mol Biol Cell. 2001; 12(4):863-879. (Biology: Western blot). View Reference
Nowak G. Protein kinase C-alpha and ERK1/2 mediate mitochondrial dysfunction, decreases in active Na+ transport, and cisplatin-induced apoptosis in renal cells. J Biol Chem. 2002; 277(45):43377-43388. (Biology: Western blot). View Reference
Reszka AA, Seger R, Diltz CD, Krebs EG, Fischer EH. Association of mitogen-activated protein kinase with the microtubule cytoskeleton. Proc Natl Acad Sci U S A. 1995; 92(19):8881-8885. (Biology: Immunofluorescence, Western blot). View Reference
Wan Y, Huang XY. Analysis of the Gs/mitogen-activated protein kinase pathway in mutant S49 cells. J Biol Chem. 1998; 273(23):14533-14537. (Biology: Immunoprecipitation). View Reference

View All (5)

610031 Rev. 1

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.