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Purified Mouse Anti-CD220
Purified Mouse Anti-CD220

Western blot analysis of CD220 on a mouse liver lysate. Lane 1: 1:250; lane 2: 1:500; lane 3: 1:1000 dilution of the anti- CD220 antibody.

Purified Mouse Anti-CD220

Immunofluorescence staining of PFSK-1 cells (human neuroectodermal tumor line; ATCC CRL-2060).

Western blot analysis of CD220 on a mouse liver lysate. Lane 1: 1:250; lane 2: 1:500; lane 3: 1:1000 dilution of the anti- CD220 antibody.

Immunofluorescence staining of PFSK-1 cells (human neuroectodermal tumor line; ATCC CRL-2060).

Product Details
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BD Transduction Laboratories™
Insulin Receptor β
Mouse (QC Testing), Rat (Tested in Development)
Mouse IgG1
Human Insulin pro-receptor β subunit 1006-1144
Western blot (Routinely Tested), Immunofluorescence, Immunoprecipitation (Tested During Development)
95 kDa
250 µg/ml
AB_397516
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
610110 Rev. 1
Antibody Details
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46/CD220

Insulin Receptor (IR) is a transmembrane receptor tyrosine kinase which, upon insulin binding, initiates a cascade of events, including autophosphorylation, phosphorylation of cellular protein substrates, glucose transport, and glycogen synthesis. IR is synthesized as a large glycosylated precursor that is cleaved upon maturation into a 130 kDa α-subunit with kinase activity and a 95 kDa β-subunit. The active Insulin Receptor is a heterotetramer of homologus α and β subunits joined by disulfide bonds. Among the major cytosolic substrates of the Insulin Receptor are IRS-1 and -2, β-Adrenergic receptor, and pp15 (adipocyte lipid-binding protein, ALBP). Autophosphorylation of the IR recruits IRS-1 and -2 to the phosphotyrosines. Subsequently, the phosphorylated IRS-1 and -2 act as docking sites for other signaling proteins like PI3-Kinase, Shc, PTP1D, Nck, etc. In addition, the phosphatase LAR is tightly associated with the IR and LAR becomes activated after insulin stimulation dephosphorylating the IR and its substrates. Therefore, LAR provides a turn-off mechanism in insulin signaling.

This antibody is routinely tested by western blot analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.

610110 Rev. 1
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
610110 Rev.1
Citations & References
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Development References (5)

  1. Ebina Y, Ellis L, Jarnagin K, et al. The human insulin receptor cDNA: the structural basis for hormone-activated transmembrane signalling. Cell. 1985; 40(4):747-758. (Biology). View Reference
  2. Frattali AL, Treadway JL, Pessin JE. Insulin/IGF-1 hybrid receptors: implications for the dominant-negative phenotype in syndromes of insulin resistance. J Cell Biochem. 1992; 48(1):43-50. (Biology). View Reference
  3. Shumay E, Song X, Wang HY, Malbon CC. pp60Src mediates insulin-stimulated sequestration of the beta(2)-adrenergic receptor: insulin stimulates pp60Src phosphorylation and activation. Mol Biol Cell. 2002; 13(11):3943-3954. (Biology: Western blot). View Reference
  4. Yip CC, Jack E. Insulin receptors are bivalent as demonstrated by photoaffinity labeling. J Biol Chem. 1992; 267(19):13131-13134. (Biology). View Reference
  5. Zundel W, Swiersz LM, Giaccia A. Caveolin 1-mediated regulation of receptor tyrosine kinase-associated phosphatidylinositol 3-kinase activity by ceramide. Mol Cell Biol. 2000; 20(5):1507-1514. (Biology: Western blot). View Reference
View All (5) View Less
610110 Rev. 1

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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

Non-IVD products are For Research Use Only. Not for use in diagnostic or therapeutic procedures.

 

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