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Purified Mouse Anti- Bcl-x
Purified Mouse Anti- Bcl-x

Western blot analysis of Bcl-x on a mouse macrophage lysate. Lane 1: 1:500, lane 2: 1:1000, lane 3: 1:2000 dilution of the mouse anti- Bcl-x antibody.

Purified Mouse Anti- Bcl-x

Immunofluorescence staining of rabbit brain.

Western blot analysis of Bcl-x on a mouse macrophage lysate. Lane 1: 1:500, lane 2: 1:1000, lane 3: 1:2000 dilution of the mouse anti- Bcl-x antibody.

Immunofluorescence staining of rabbit brain.

Product Details
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BD Transduction Laboratories™
Mouse (QC Testing), Rat, Chicken (Tested in Development)
Mouse IgG2b
Rat Bcl-xL aa. 18-233
Western blot (Routinely Tested), Immunofluorescence, Immunohistochemistry (Tested During Development), Immunoprecipitation (Not Recommended)
26 kDa
250 µg/ml
AB_397609
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.

Recommended Assay Procedures

Western blot:  Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Antibody Details
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4/Bcl-x

Bcl-x is related to the Bcl-2 protein and can function independently of Bcl-2 in regulating apoptosis (programmed cell death).  The bcl-x transcript is found in a number of tissues, with the highest levels in the lymphoid and central nervous systems.  Two distinct cDNA species, bcl-xL and bcl-xS, have been observed and appear to arise from alternate 5' splice sites located within the first coding exon of the bcl-x gene.  Bcl-xL is composed of 233 amino acids and is similar in size and structure to Bcl-2.  Bcl-xS lacks 63 amino acids corresponding to the region of Bcl-xL with the highest degree of homology to Bcl-2.  Like Bcl-2, Bcl-xL has been reported to inhibit cell death upon growth factor withdrawal in an IL-3-dependent cell line.   However, Bcl-xS inhibits the ability of Bcl-2 to enhance cell survival in the absence of growth factors.

This antibody is routinely tested by western blot analysis.  Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.

Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
Citations & References
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Development References (5)

  1. Anderson JS, Teutsch M, Dong Z, Wortis HH. An essential role for Bruton's [corrected] tyrosine kinase in the regulation of B-cell apoptosis. Proc Natl Acad Sci U S A. 1996; 93(26):10966-10971. (Biology: Western blot). View Reference
  2. Imaizumi K, Morihara T, Mori Y. The cell death-promoting gene DP5, which interacts with the BCL2 family, is induced during neuronal apoptosis following exposure to amyloid beta protein. J Biol Chem. 1999; 274(12):7975-7981. (Biology: Immunoprecipitation, Western blot). View Reference
  3. Kurowska M, Rudnicka W, Kontny E. Fibroblast-like synoviocytes from rheumatoid arthritis patients express functional IL-15 receptor complex: endogenous IL-15 in autocrine fashion enhances cell proliferation and expression of Bcl-x(L) and Bcl-2. J Immunol. 2002; 169(4):1760-1767. (Biology: Flow cytometry). View Reference
  4. Rosen K, Coll ML, Li A, Filmus J. Transforming growth factor-alpha prevents detachment-induced inhibition of c-Src kinase activity, Bcl-XL down-regulation, and apoptosis of intestinal epithelial cells.. 2001; 276(40):37273-37279. (Biology: Western blot). View Reference
  5. Slupianek A, Hoser G, Majsterek I. Fusion tyrosine kinases induce drug resistance by stimulation of homology-dependent recombination repair, prolongation of G(2)/M phase, and protection from apoptosis. Mol Cell Biol. 2002; 22(12):4189-4201. (Biology: Flow cytometry, Western blot). View Reference
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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

Non-IVD products are For Research Use Only. Not for use in diagnostic or therapeutic procedures.

 

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