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Purified Mouse Anti-Annexin VI
Purified Mouse Anti-Annexin VI

Western blot analysis of Annexin VI on a Jurkat cell lysate (Human T-cell leukemia; ATCC TIB-152). Lane 1: 1:5000, lane 2: 1:10,000, lane 3: 1:20,000 dilution of the mouse anti-Annexin VI antibody.

Purified Mouse Anti-Annexin VI

Immunofluorescence staining of WI-38 cells (Human lung fibroblasts; ATCC CCL-75).

Western blot analysis of Annexin VI on a Jurkat cell lysate (Human T-cell leukemia; ATCC TIB-152). Lane 1: 1:5000, lane 2: 1:10,000, lane 3: 1:20,000 dilution of the mouse anti-Annexin VI antibody.

Immunofluorescence staining of WI-38 cells (Human lung fibroblasts; ATCC CCL-75).

Product Details
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BD Transduction Laboratories™
Human (QC Testing), Rat (Tested in Development)
Mouse IgG1
Human Annexin VI aa. 1-673
Western blot (Routinely Tested), Immunofluorescence, Immunoprecipitation (Tested During Development), Immunohistochemistry (Not Recommended)
70 kDa
250 µg/ml
AB_397695
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at -20°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Recommended Assay Procedures

Western blot:  Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
610301 Rev. 1
Antibody Details
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73/Annexin VI

Annexins are a widely expressed family of Ca[2+] and phospholipid-binding proteins. At least ten have been identified in mammalian tissues. They have also been identified in Drosophila, Hydra, and Dictyostelium. Annexin VI is a 70 kDa member of the annexin family. While most of the other annexin family members have a four amino acid sequence repeat, Annexin VI displays an eight residue repeat. It is a widely expressed protein, found in lymphocytes, neurons, and many other cell types. Work on annexins has addressed the possible role of Annexin VI in various stages of the endocytic pathway. Some studies suggest that Annexin VI is required for coated-vesicle budding. However, other research directly contradicts this data. Thus, the role of Annexin VI is still under investigation.

610301 Rev. 1
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
610301 Rev.1
Citations & References
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Development References (5)

  1. Babiychuk EB, Draeger A. Annexins in cell membrane dynamics. Ca(2+)-regulated association of lipid microdomains. J Cell Biol. 2000; 150(5):1113-1124. (Biology: Immunofluorescence, Western blot). View Reference
  2. Babiychuk EB, Palstra RJ, Schaller J, Kampfer U, Draeger A. Annexin VI participates in the formation of a reversible, membrane-cytoskeleton complex in smooth muscle cells. J Biol Chem. 1999; 274(49):35191-35195. (Biology: Immunohistochemistry, Western blot). View Reference
  3. Chen JS, Coustan-Smith E, Suzuki T. Identification of novel markers for monitoring minimal residual disease in acute lymphoblastic leukemia. Blood. 2001; 97(7):2115. (Biology: Flow cytometry). View Reference
  4. Crompton MR, Moss SE, Crumpton MJ. Diversity in the lipocortin/calpactin family. Cell. 1988; 55(1):1-3. (Biology). View Reference
  5. Yu W, Cassara J, Weller PF. Phosphatidylinositide 3-kinase localizes to cytoplasmic lipid bodies in human polymorphonuclear leukocytes and other myeloid-derived cells. Blood. 2000; 95(3):1078-1085. (Biology: Western blot). View Reference
View All (5) View Less
610301 Rev. 1

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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

Non-IVD products are For Research Use Only. Not for use in diagnostic or therapeutic procedures.

 

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