Skip to main content Skip to navigation
Purified Mouse Anti-Adenovirus Type 5 E1A
Purified Mouse Anti-Adenovirus Type 5 E1A

Western blot analysis of adenovirus E1A. Lysates from 293 human embryonic kidney cells were probed with anti-adenovirus Type 5 E1A (clone M58, Cat. No. 14161A). The E1A protein is identified as a doublet at ~35-46 kDa.

Western blot analysis of adenovirus E1A. Lysates from 293 human embryonic kidney cells were probed with anti-adenovirus Type 5 E1A (clone M58, Cat. No. 14161A). The E1A protein is identified as a doublet at ~35-46 kDa.

Product Details
Down Arrow Up Arrow


BD Pharmingen™
Human (QC Testing)
Mouse IgG2a
A trpE-E1A fusion protein
Western blot (Routinely Tested), Immunofluorescence, Immunoprecipitation (Reported)
0.5 mg/ml
AB_395274
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C.

Recommended Assay Procedures

Applications include western blot analysis (1-2 µg/ml), immunoprecipitation (1-2 µg/one million cells) 4and indirect immunofluorescence of cultured cells (5-20 µg/ml). The E1A proteins migrate at various molecular weights depending on mRNA processing and posttranslational modifications; at least 60 E1A polypeptide species have been identified, ranging between 30 and 46 kDa. Two species resulting from the 12S and 13S E1A mRNAs have reduced molecular weights of 26 kDa and 32 kDa, respectively. 293 human embryonic kidney cells or E1A/Adenovirus Type 5 infected HeLa cells are suggested as positive controls.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
554155 Rev. 6
Antibody Details
Down Arrow Up Arrow
M58

Human adenoviruses are widely used as models for studying cellular transformation and regulation of gene expression. The adenovirus E1A region plays a central role in each of these processes. This region of the viral genome encodes a series of related proteins with multifunctional capabilities. E1A regulates the transcriptional regulation of a wide variety of viral and cellular promoters. Most commonly, this results in the activation of transcription from the target promoter. In other cases, transcription from the targeted promoter is repressed by the action of E1A. E1A proteins also contribute to the transforming capabilities of adenoviruses. The E1A and E1B regions together comprise the transforming region of adenovirus. While expression of E1A alone is sufficient to immortalize primary cells, complete transformation requires the additional expression of the E1B region. Several other oncogenes, including the activated H-ras oncogene and polyoma middle T antigen, can complement E1A by substituting for E1B in transformation assays. Similarly, E1A can be replaced by polyoma large T antigen, c-myc and mutated p53 protein. Several conserved regions of E1A are strikingly similar to portions of other viral oncoproteins, e.g., HPV-16, HPV-18 E7 and SV40 large T antigen. Like HPV E7 and SV40 large T antigen, E1A proteins can form a specific complex with the retinoblastoma tumor suppressor gene product (Rb protein). Complex formation between the products of oncogenes and tumor suppressor genes are believed to be important in cellular transformation. The exact mechanism(s) of these complexes are not fully understood, however they may be involved in negative regulation of cellular growth and/or differentiation. M58 recognizes Adenovirus Type 5 E1A proteins.4 A trpE-E1A fusion protein was used as immunogen. The fusion protein contained the C-terminal 266 amino acids of the 13S E1A protein. This is about 90% of the E1A 13S coding sequence.

554155 Rev. 6
Format Details
Down Arrow Up Arrow
Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
554155 Rev.6
Citations & References
Down Arrow Up Arrow

Development References (5)

  1. Adenovirus. In: Hesketh R. The Oncogene Handbook. New York: Academic Press; 1994:135-147.
  2. Berk AJ. Adenovirus promoters and E1A transactivation. Annu Rev Genet. 1986; 20:45-79. (Biology). View Reference
  3. Harlow E, Franza BR Jr, Schley C. Monoclonal antibodies specific for adenovirus early region 1A proteins: extensive heterogeneity in early region 1A products. J Virol. 1985 Sepetember; 55(3):533-546. (Clone-specific: Immunofluorescence, Immunoprecipitation). View Reference
  4. Klein G. The approaching era of the tumor suppressor genes. Science. 1987 December; 238(4833):1539-1545. (Biology). View Reference
  5. Whyte P, Williamson NM, Harlow E. Cellular targets for transformation by the adenovirus E1A proteins. Cell. 1989 January; 56(1):67-75. (Biology). View Reference
View All (5) View Less
554155 Rev. 6

Please refer to Support Documents for Quality Certificates


Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

Non-IVD products are For Research Use Only. Not for use in diagnostic or therapeutic procedures.

 

23-22940-00