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Purified Mouse Anti-Human CD253
Purified Mouse Anti-Human CD253

Western blot analysis of TRAIL. WI-38 human diploid fibroblasts probed with anti-human TRAIL (clone B35-1,). The B35-1 antibody identifies TRAIL as an ~32 kDa band.

Western blot analysis of TRAIL. WI-38 human diploid fibroblasts probed with anti-human TRAIL (clone B35-1,). The B35-1 antibody identifies TRAIL as an ~32 kDa band.

Product Details
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BD Pharmingen™
TRAIL, APO-2L, TL2
Human (QC Testing)
Mouse IgG2b, κ
Recombinant Human TRAIL protein
Western blot (Routinely Tested), Immunochemistry (Reported)
32 kDa (surface), 28 kDa (soluble)
0.5 mg/ml
AB_396431
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C.

Recommended Assay Procedures

WI-38 human diploid fibroblasts (ATCC CC L75) are suggested as a positive control for Western blot analysis.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
556468 Rev. 5
Antibody Details
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B35-1

TRAIL (TNF-Related Apoptosis-Inducing Ligand) also known as Apo2L is a member of the TNF ligand family. TRAIL is a type II membrane protein which may be expressed as full-length, cell surface associated protein as well as in a  soluble form. Both surface and soluble forms of TRAIL rapidly induce apoptosis on a wide range of cell lines. TRAIL-mediated apoptosis has been shown to involve the activation of ICE-related proteases/caspases, and is blocked by over-expression of the Ced-3 protease inhibitor, CrmA.  TRAIL has also been reported to induce the transcription factor, NF-κB, in a cell typespecific manner.  Three TRAIL receptors, DR4, DR5 and DcR1/TRID have been identified. Surface  human TRAIL migrates at ~32 kDa by SDS/PAGE, soluble, human recombinant TRAIL migrates at 28 kDa by SDS/PAGE and has also been observed in solution as a multimeric, 80 kDa form.

The B35-1 antibody recognizes human TRAIL.  A recombinant human TRAIL protein fragment corresponding to amino acids 32-115 was used as immunogen. TRAIL has been renamed as CD253 recently.

556468 Rev. 5
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
556468 Rev.5
Citations & References
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Development References (6)

  1. Mariani SM, Matiba B, Armandola EA, Krammer PH. Interleukin 1 beta-converting enzyme related proteases/caspases are involved in TRAIL-induced apoptosis of myeloma and leukemia cells. J Cell Biol. 1997; 137(1):221-229. (Biology). View Reference
  2. Marsters SA, Pitti RM, Donahue CJ, Ruppert S, Bauer KD, Ashkenazi A. Activation of apoptosis by Apo-2 ligand is independent of FADD but blocked by CrmA. Curr Biol. 1996; 6(6):750-752. (Biology). View Reference
  3. Pitti RM, Marsters SA, Ruppert S, Donahue CJ, Moore A, Ashkenazi A. Induction of apoptosis by Apo-2 ligand, a new member of the tumor necrosis factor cytokine family. J Biol Chem. 1996; 271(22):12687-12690. (Biology). View Reference
  4. Sheridan JP, Marsters SA, Pitti RM, et al. Control of TRAIL-induced apoptosis by a family of signaling and decoy receptors. Science. 1997; 277(5327):818-821. (Biology). View Reference
  5. Wang Q, Wang X, Hernandez A, Hellmich MR, Gatalica Z, Evers BM. Regulation of TRAIL expression by the phosphatidylinositol 3-kinase/Akt/GSK-3 pathway in human colon cancer cells. J Biol Chem. 2002; 277(39):36602-36610. (Clone-specific: Immunohistochemistry). View Reference
  6. Wiley SR, Schooley K, Smolak PJ, et al. Identification and characterization of a new member of the TNF family that induces apoptosis. Immunity. 1995; 3(6):673-682. (Biology). View Reference
View All (6) View Less
556468 Rev. 5

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