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Purified Mouse Anti-Human CD253
Purified Mouse Anti-Human CD253

Western blot analysis of TRAIL. WI-38 human diploid fibroblasts probed with anti-human TRAIL (clone B35-1,). The B35-1 antibody identifies TRAIL as an ~32 kDa band.

Western blot analysis of TRAIL. WI-38 human diploid fibroblasts probed with anti-human TRAIL (clone B35-1,). The B35-1 antibody identifies TRAIL as an ~32 kDa band.

Product Details
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BD Pharmingen™
Human (QC Testing)
Mouse IgG2b, κ
Recombinant Human TRAIL protein
Western blot (Routinely Tested), Immunochemistry (Reported)
32 kDa (surface), 28 kDa (soluble)
0.5 mg/ml
Aqueous buffered solution containing ≤0.09% sodium azide.

Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C.

Recommended Assay Procedures

WI-38 human diploid fibroblasts (ATCC CC L75) are suggested as a positive control for Western blot analysis.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
556468 Rev. 5
Antibody Details
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TRAIL (TNF-Related Apoptosis-Inducing Ligand) also known as Apo2L is a member of the TNF ligand family. TRAIL is a type II membrane protein which may be expressed as full-length, cell surface associated protein as well as in a  soluble form. Both surface and soluble forms of TRAIL rapidly induce apoptosis on a wide range of cell lines. TRAIL-mediated apoptosis has been shown to involve the activation of ICE-related proteases/caspases, and is blocked by over-expression of the Ced-3 protease inhibitor, CrmA.  TRAIL has also been reported to induce the transcription factor, NF-κB, in a cell typespecific manner.  Three TRAIL receptors, DR4, DR5 and DcR1/TRID have been identified. Surface  human TRAIL migrates at ~32 kDa by SDS/PAGE, soluble, human recombinant TRAIL migrates at 28 kDa by SDS/PAGE and has also been observed in solution as a multimeric, 80 kDa form.

The B35-1 antibody recognizes human TRAIL.  A recombinant human TRAIL protein fragment corresponding to amino acids 32-115 was used as immunogen. TRAIL has been renamed as CD253 recently.

556468 Rev. 5
Format Details
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Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
556468 Rev.5
Citations & References
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Development References (6)

  1. Mariani SM, Matiba B, Armandola EA, Krammer PH. Interleukin 1 beta-converting enzyme related proteases/caspases are involved in TRAIL-induced apoptosis of myeloma and leukemia cells. J Cell Biol. 1997; 137(1):221-229. (Biology). View Reference
  2. Marsters SA, Pitti RM, Donahue CJ, Ruppert S, Bauer KD, Ashkenazi A. Activation of apoptosis by Apo-2 ligand is independent of FADD but blocked by CrmA. Curr Biol. 1996; 6(6):750-752. (Biology). View Reference
  3. Pitti RM, Marsters SA, Ruppert S, Donahue CJ, Moore A, Ashkenazi A. Induction of apoptosis by Apo-2 ligand, a new member of the tumor necrosis factor cytokine family. J Biol Chem. 1996; 271(22):12687-12690. (Biology). View Reference
  4. Sheridan JP, Marsters SA, Pitti RM, et al. Control of TRAIL-induced apoptosis by a family of signaling and decoy receptors. Science. 1997; 277(5327):818-821. (Biology). View Reference
  5. Wang Q, Wang X, Hernandez A, Hellmich MR, Gatalica Z, Evers BM. Regulation of TRAIL expression by the phosphatidylinositol 3-kinase/Akt/GSK-3 pathway in human colon cancer cells. J Biol Chem. 2002; 277(39):36602-36610. (Clone-specific: Immunohistochemistry). View Reference
  6. Wiley SR, Schooley K, Smolak PJ, et al. Identification and characterization of a new member of the TNF family that induces apoptosis. Immunity. 1995; 3(6):673-682. (Biology). View Reference
View All (6) View Less
556468 Rev. 5

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