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Purified Mouse Anti-Rat IgG2a
Product Details
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BD Pharmingen™
Rat (QC Testing)
Mouse BALB/c IgG1, κ
Pooled Rat IgG mAbs
ELISA Capture (Routinely Tested)
0.5 mg/ml
AB_395134
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C.

Recommended Assay Procedures

This antibody has been tested by ELISA (~2 µg/ml) to assure specificity and reactivity. For the sandwich rat IgG2a ELISA, purified B46-7 mAb is optimal for capture with biotinylated anti-rat IgG2a RG7/1.30 mAb (Cat. No. 553894) for detection.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use.
553918 Rev. 5
Antibody Details
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B46-7

The B46-7 antibody reacts specifically with rat IgG2a. It does not react with other rat Ig isotypes, nor with rabbit, hamster, guinea pig, or human immunoglobulins.

553918 Rev. 5
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
553918 Rev.5

Please refer to Support Documents for Quality Certificates


Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

Non-IVD products are For Research Use Only. Not for use in diagnostic or therapeutic procedures.

 

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