ELISA Capture: Purified Mouse Anti-Human CD154 antibody (Cat. No. 552559) is useful as a capture antibody for a sandwich ELISA for measuring human CD40L protein levels. Purified Mouse Anti-Human CD154 antibody can be paired with Biotin Mouse Anti-Human CD154 (Cat. No. 552560) as the detecting antibody. Purified Mouse Anti-Human CD154 antibody should be titrated 1 - 4 µg/ml to determine the optimal concentration for ELISA capture. To obtain linear standard curves, doubling dilutions of recombinant human soluble CD40L, ranging from 2000 to 15 pg/ml are recommended for inclusion in each ELISA plate. For specific methodology see the "ELISA and ELISPOT" section at our website: http://www.bdbiosciences.com/en-us/support/bd-biosciences-resource-library.
Note: This ELISA antibody pair shows no cross-reactivity with the following recombinant human proteins: CD40, IL-1Ra, IL-1R1, IL-1RII, IL-1α, IL-1β, IL-2, IL-3, IL-4, sIL-4R, IL-5, IL-6, IL-6R, IL-7, IL-8, IL-10, IL-12p40, IL-12p70, IL-13, IL-15, TNF-α, TNF-β, sTNFRI, sTNFRII, IFNγ, TRAIL, MCSF, TGFβ, Trx