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Biotin Rat Anti-Human CD124
Product Details
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BD Pharmingen™
IL-4 Receptor α chain, CD124
Human (QC Testing)
Rat IgG1, κ
ELISA Detection (Routinely Tested)
0.5 mg/ml
AB_394397
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed. Store undiluted at 4°C.

Recommended Assay Procedures

ELISA Detection: Biotinylated hIL4R-M8.2.2 (Cat. No. 552473) serves as the detection antibody in a sandwich ELISA for measuring human soluble IL-4R protein levels. Biotinylated hIL4R-M8.2.2 antibody can be paired with the purified hIL4R-M10.1 anti-human IL-4R (Cat No. 552472) using recombinant human soluble IL-4R as the standard. This detection antibody should be titrated between 0.5 - 1 µg/ml to determine its optimal concentration for ELISA detection.  To obtain linear standard curves, doubling dilutions of recombinant soluble human IL-4R ranging from 2000 to 15 pg/ml are recommended for inclusion in each ELISA plate. For specific methodology, see BD Pharmingen's Immune Function Handbook, which is posted on our website at www.BDBiosciences.com.

Note: This ELISA antibody pair shows no cross-reactivity with the following recombinant human cytokines: IL-1RI, IL-1RII, IL-1α, IL-1ß, IL-2, IL-2sR-α, IL-3, IL-4, IL-5, IL-6, IL-6R gp130, IL-7, IL-8, IL-10, IL-12 p40, IL-12 p70, IL-13, IL-15, TNF-α, TNF-ß, sTNFRI, sTNFRII, GM-CSF, TGFß, IFN-ß, CD40L, TRAIL, FasL.

Product Notices

  1. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  2. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
552473 Rev. 1
Antibody Details
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hIL4R-M8.2.2

The soluble human IL-4 receptor, also known as sCD124, is a140-kDa multimeric membrane glycoprotein that binds IL-4 with high affinity. Although many cell types are able to produce soluble IL-4R at low levels, its production is significantly up-regulated in vitro by T cell activation and IL-4. Cytokines secreted by Th2 cells were found to significantly up-regulate soluble IL-4R production following antigenic stimulation. Studies have indicated that the production of sIL-4R in vivo is closely associated with the secretion of IL-4, and are consistent with reports indicating that sIL-4R are involved in the regulation of IL-4 activity during immune response.

552473 Rev. 1
Format Details
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Biotin
Biotin is a ubiquitous co-factor (also known as Vitamin B7) that has many properties that make it extremely useful for molecular biology. Biotin has an extremely high affinity for the Avidin family of proteins (Kd = 10-15 M), making it the perfect tool to link two molecules. Biotin labeled antibodies can be combined with any number of Avidin-conjugated probes in order to customize an assay to a particular need. This is especially useful in the case of magnetic cell separation using streptavidin/magnetic bead conjugates, or in the case of flow cytometry using streptavidin/fluorophore conjugates.
Biotin
552473 Rev.1
Citations & References
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Development References (3)

  1. Chomarat P, Banchereau J. An update on interleukin-4 and its receptor. Eur Cytokine Netw. 1997; 8(4):333-344. (Biology). View Reference
  2. Fernandez-Botran R, Chilton PM, Ma Y, Windsor JL, Street NE. Control of the production of soluble interleukin-4 receptors: implications in immunoregulation. J Leukoc Biol. 1996; 59(4):499-504. (Biology). View Reference
  3. Jacobs CA, Lynch DH, Roux ER, et al. Characterization and pharmacokinetic parameters of recombinant soluble interleukin-4 receptor. Blood. 1991; 77(11):2396-2403. (Biology). View Reference
552473 Rev. 1

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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

Non-IVD products are For Research Use Only. Not for use in diagnostic or therapeutic procedures.

 

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