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Biotin Mouse Anti-Human sCD14
Product Details
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BD Pharmingen™
sCD14
Human (QC Testing)
Mouse IgG2a, κ
Soluble Human CD14
ELISA Detection (Routinely Tested)
0.5 mg/ml
AB_394188
Aqueous buffered solution containing protein stabilizer and ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.

Recommended Assay Procedures

ELISA Detection: The Biotinylated 3-C39 (Cat. No. 551405) is useful as a detector antibody for a sandwich ELISA for measuring human soluble CD14 protein levels. Biotinylated 3-C39 can be paired with purified 55-3 anti-human sCD14 (Cat. No. 551403) as the capture antibody, with recombinant soluble CD14 as the standard. The detection antibody should be titrated between 0.5 - 1 µg/ml to determine its optimal concentration for ELISA detection. To obtain linear standard curves, doubling dilutions of recombinant human soluble CD14, ranging from 2000 to 15 pg/ml are recommended for inclusion in each ELISA plate. For specific methodology, please visit the chapter on ELISA in the Immune Function Handbook, which is posted on our web site, www.bdbiosciences.com.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
551405 Rev. 2
Antibody Details
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3-C39

The 3-C39 monoclonal antibody reacts with human CD14. CD14 is a 53-55kDa cell surface glycoprotein that is expressed by mature monocytes, macrophages, and activated granulocytes as a glycophosphatidylinositol-linked protein. CD14 functions as a receptor for lipopolysaccharide (LPS, endotoxin) when LPS is bound to LBP (LPS binding protein). Soluble forms of CD14 (sCD14) can be detected either in serum or in supernatants from cell cultures. Soluble CD14 is produced either by shedding of membrane CD14 or by release from cells before addition of the GPI anchor. Both membrane and sCD14 function to enhance cell activation by LPS. Binding of LPS-LBP complexes to CD14 activates monocytes or macrophages to produce and secrete cytokines and pro-inflammatory mediators. Non-CD14 expressing cells, such as endothelial cells, can be activated by a complex of LPS and sCD14. Serum levels of sCD14 increase in association with sepsis, infectious disease, autoimmunity and allergic disorders.

551405 Rev. 2
Format Details
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Biotin
Biotin is a ubiquitous co-factor (also known as Vitamin B7) that has many properties that make it extremely useful for molecular biology. Biotin has an extremely high affinity for the Avidin family of proteins (Kd = 10-15 M), making it the perfect tool to link two molecules. Biotin labeled antibodies can be combined with any number of Avidin-conjugated probes in order to customize an assay to a particular need. This is especially useful in the case of magnetic cell separation using streptavidin/magnetic bead conjugates, or in the case of flow cytometry using streptavidin/fluorophore conjugates.
Biotin
551405 Rev.2
Citations & References
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Development References (10)

  1. Arditi M, Zhou J, Dorio R, Rong GW, Goyert SM, Kim KS. Endotoxin-mediated endothelial cell injury and activation: role of soluble CD14. Infect Immun. 1993; 61(8):3149-3156. (Biology). View Reference
  2. Egerer K, Feist E, Rohr U, Pruss A, Burmester GR, Dorner T. Increased serum soluble CD14, ICAM-1 and E-selectin correlate with disease activity and prognosis in systemic lupus erythematosus. Lupus. 2000; 9(8):614-621. (Biology). View Reference
  3. Ferrero E, Goyert SM. Nucleotide sequence of the gene encoding the monocyte differentiation antigen, CD14. Nucleic Acids Res. 1988; 16(9):4173. (Biology). View Reference
  4. Goyert SM, Ferrero E, Rettig WJ, Yenamandra AK, Obata F, Le Beau MM. The CD14 monocyte differentiation antigen maps to a region encoding growth factors and receptors. Science. 1988; 239(4839):497-500. (Biology). View Reference
  5. Goyert SM, Lohen L, Gangloff SC, Ashmun R, Haeffner Cavaillon N. CD14 Workshop Panel report. In: Kishimoto T. Tadamitsu Kishimoto .. et al., ed. Leucocyte typing VI : white cell differentiation antigens : proceedings of the sixth international workshop and conference held in Kobe, Japan, 10-14 November 1996. New York: Garland Pub.; 1997:36.
  6. Haziot A, Chen S, Ferrero E, Low MG, Silber R, Goyert SM. The monocyte differentiation antigen, CD14, is anchored to the cell membrane by a phosphatidylinositol linkage. J Immunol. 1988; 141(2):547-552. (Biology). View Reference
  7. Haziot A, Rong GW, Lin XY, Silver J, Goyert SM. Recombinant soluble CD14 prevents mortality in mice treated with endotoxin (lipopolysaccharide). J Immunol. 1995; 154(12):6529-6532. (Biology). View Reference
  8. Haziot A, Rong GW, Silver J, Goyert SM. Recombinant soluble CD14 mediates the activation of endothelial cells by lipopolysaccharide. J Immunol. 1993; 151(3):1500-1507. (Biology). View Reference
  9. Nockher WA, Wick M, Pfister HW. Cerebrospinal fluid levels of soluble CD14 in inflammatory and non-inflammatory diseases of the CNS: upregulation during bacterial infections and viral meningitis. J Neuroimmunol. 1999; 101(2):161-169. (Biology). View Reference
  10. Ulevitch RJ, Tobias PS. Receptor-dependent mechanisms of cell stimulation by bacterial endotoxin. Annu Rev Immunol. 1995; 13:437-457. (Biology). View Reference
View All (10) View Less
551405 Rev. 2

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