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Rat TNF Standard
Product Details
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BD™ Cytometric Bead Array (CBA)
Flow cytometry (Routinely Tested)
AB_2869208
RUO


Preparation And Storage

Store the lyophilized standard at 4°C prior to reconstitution.

Lyosphere(s) from 1 vial of Rat TNF Standard, when reconstituted in 4.0 mL Assay Diluent, approximates the CBA activity of Rat TNF at a concentration of 10,000 pg/mL. Following reconstitution, maintain the Rat TNF at 4°C and use within 12 hours. Any unused reconstituted standard must be discarded after this period (do not store or reuse).

Recommended Assay Procedures

The Rat TNF Standard has been tested to assure to function as a standard when used with the BD™ Cytometric Bead Array (CBA), such as the BD™ CBA Rat TNF Flex Set (Cat. No. 558309).  Investigators are encouraged to refer to the technical data sheet for the BD™ CBA Rat TNF Flex Set (Cat. No. 558309).  An abbreviated instruction has been provided below (Preparation of Rat TNF Standard Protocol).  The Rat TNF Standard should be reconstituted using Assay Diluent, which is provided with the BD™ CBA Mouse/Rat Soluble Protein Master Buffer Kit (Cat. No. 558266 or 558267) or it may also be purchased separately (Cat. No. 560104).

Preparation of Rat TNF Standard:  The Rat TNF Standard is lyophilized and should be reconstituted and serially diluted before mixing with CBA capture beads and PE detection reagents.

1.  Open one vial of the lyophilized Rat TNF Standard. Transfer the lyosphere(s) to a polypropylene tube (BD Falcon™, Cat. No. 352097). Label the tube "Top Standard".

2.  Reconstitute the standard with 4.0 mL of Assay Diluent. Allow the reconstituted standard to equilibrate for at least 15 minutes before making dilutions. Mix the reconstituted protein by pipette only. Do not vortex or mix vigorously.

3.  Label additional 12 x 75 mm tubes (BD Falcon™, Cat. No. 352008) and arrange them in the following order: Top Standard, 1:2, 1:4, 1:8, 1:16, 1:32, 1:64, 1:128, and 1:256.

4.  Add 500 µl of Assay Diluent to each of the dilution tubes.

5.  Perform a serial dilution by transferring 500 µl from the Top Standard to the 1:2 dilution tube and mix thoroughly. Do not vortex, mix by pipet only. Continue making serial dilutions by transferring 500 µl from the 1:2 tube to the 1:4 tube and so on to the 1:256 tube and mix thoroughly. Prepare one tube containing only Assay Diluent to serve as the 0 pg/mL negative control.

Product Notices

  1. ProClin is a trademark of Rohm and Haas Company.
  2. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  3. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  4. Warning: CBA lyophilized standard contains 0.02% (w/w) and Detection Reagent (Part A) contains 0.002% (w/w) of a CMIT/MIT mixture (3:1), which is a mixture of: 5-chloro-2-methyl-4-isothiazolin-3-one [EC No 247-500-7] and 2-methyl-4-isothiazolin-3-one [EC No 220-239-6] (3:1).Hazard statement: May cause an allergic skin reaction.Precautionary statements: Contaminated work clothing should not be allowed out of the workplace. Wear protective gloves/eye/face protection. Wear protective clothing. Avoid breathing mist/vapours/spray. If skin irritation or rash occurs: Get medical advice/attention. IF ON SKIN: Wash with plenty of water. Take off contaminated clothing and wash it before reuse. Dispose of contents/container in accordance with local/regional/national/international regulations.
558484 Rev. 5
Antibody Details
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This product contains lyophilized recombinant rat tumor necrosis factor (TNF). When reconstituted, this product can be used as a standard control with the BD™ CBA Rat TNF Flex Set (Cat. No. 558309). Please refer to the technical data sheet included with the BD™ CBA Rat TNF Flex Set for further information.

558484 Rev. 5

Please refer to Support Documents for Quality Certificates


Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

Non-IVD products are For Research Use Only. Not for use in diagnostic or therapeutic procedures.

 

23-22940-00