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Purified NA/LE Rat Anti-Mouse CD90
Product Details
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BD Pharmingen™
Thy-1
Mouse (QC Testing)
Rat LEW, also known as Lewis IgG2c, κ
Mouse T-cell hybridoma C 6/68
Flow cytometry (Routinely Tested), Immunohistochemistry-frozen (Tested During Development), (Co)-stimulation, Activation, Apoptosis, Immunohistochemistry-zinc-fixed (Reported), Immunohistochemistry-formalin (antigen retrieval required) (Not Recommended)
1.0 mg/ml
No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2µm sterile filtered. Endotoxin level is ≤0.01 EU/µg (≤0.001 ng/µg) of protein as determined by the LAL assay.
RUO


Preparation And Storage

Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. This preparation contains no preservatives, thus it should be handled under aseptic conditions.

Recommended Assay Procedures

Other reported applications include in vitro T-cell activation induction of apoptosis, and immunohistochemical staining (IHC) of acetone-fixed frozen and zinc-fixed paraffin-embedded sections. IHC of formalin-fixed paraffin-embedded sections is not recommended. For IHC of tissue expressing the Thy-1.2 alloantigen, investigators are encouraged to use Rat Anti-Mouse CD90.2 mAb 53-2.1 (Cat. no. 550543).

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
553015 Rev. 14
Antibody Details
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G7

The G7 antibody reacts with the Thy-1.1 and Thy-1.2 alloantigens (CD90.1 and CD90.2, respectively) on thymocytes, peripheral T lymphocytes, some intraepithelial T lymphocytes (IEL, DEC), and neurons, but not B lymphocytes, of all mouse strains.  CD90 is a GPI-anchored membrane glycoprotein of the Ig superfamily which is involved in signal transduction.  In addition, there is evidence that CD90 mediates adhesion of thymocytes to thymic stroma.  Soluble G7 mAb stimulates murine T-cell proliferation and IL-2 secretion, via signalling through the TCR/CD3 complex.  mAb G7 has been reported to promote apoptosis of thymocytes and CTL clones, as determined by morphological and biochemical criteria.  The Thy-1-triggered thymocyte death was found to be developmentally regulated and targeted mainly within the CD4+CD8+CD3- and CD4+CD8+CD3lo cultured immature thymocytes.  G7 antibody does not react with Thy-1 (CD90) on rat thymocytes.

553015 Rev. 14
Format Details
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NA/LE
NA/LE refers to the culture and purification methods and buffer used to produce purified antibodies with no azide and low endotoxin: Aqueous buffered solution containing no preservative, 0.2µm sterile filtered. Endotoxin level is ≤0.01 EU/µg (≤0.001 ng/µg) of protein as determined by the LAL assay.NA/LE are perfectly suited to be used in culture or in vivo (for nonhuman studies) for functional assays — blocking, neutralizing, activation or depletion — where the presence of azide may damage cells or exogenous endotoxin may signal or activate cells.
NA/LE
553015 Rev.14
Citations & References
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Development References (11)

  1. Aguilar LK, Aguilar-Cordova E, Cartwright J Jr, Belmont JW. Thymic nurse cells are sites of thymocyte apoptosis. J Immunol. 1994; 152(6):2645-2651. (Clone-specific: Immunohistochemistry). View Reference
  2. Gunter KC, Malek TR, Shevach EM. T cell-activating properties of an anti-Thy-1 monoclonal antibody. Possible analogy to OKT3/Leu-4. J Exp Med. 1984; 159(3):716-730. (Immunogen: Activation). View Reference
  3. He HT, Naquet P, Caillol D, Pierres M. Thy-1 supports adhesion of mouse thymocytes to thymic epithelial cells through a Ca2(+)-independent mechanism. J Exp Med. 1991; 173(2):515-518. (Clone-specific: Activation). View Reference
  4. Hueber AO, Raposo G, Pierres M, He HT. Thy-1 triggers mouse thymocyte apoptosis through a bcl-2-resistant mechanism. J Exp Med. 1994; 179(3):785-796. (Clone-specific: Apoptosis). View Reference
  5. Kroczek RA, Gunter KC, Germain RN, Shevach EM. Thy-1 functions as a signal transduction molecule in T lymphocytes and transfected B lymphocytes. Nature. 1986; 322(6075):181-184. (Biology). View Reference
  6. Kruisbeek AM, Shevach EM. Proliferative assays for T cell function. Curr Protoc Immunol. 2004; 3:3.12.1-3.12.14. (Clone-specific: Activation). View Reference
  7. Nitta H, Munger W, Wilson E. Improved in situ immunodetection of leukocytes paraffin embedded mouse spleen. Cell Vis. 1997; 4:73-80. (Clone-specific: Immunohistochemistry).
  8. Sugiyama E, Cantagrel A, Reno T, Stafford-Brady F, Yeh ET, Bonventre JV. Selection of T cell receptor expression mutants through the functionally linked Ly-6A. Cell Immunol. 1990; 130(2):271-280. (Clone-specific: Apoptosis). View Reference
  9. Tentori L, Pardoll DM, Zuniga JC . Proliferation and production of IL-2 and B cell stimulatory factor 1/IL-4 in early fetal thymocytes by activation through Thy-1 and CD3. J Immunol. 1988; 140(4):1089-1094. (Clone-specific: Activation). View Reference
  10. Ucker DS, Meyers J, Obermiller PS. Activation-driven T cell death. II. Quantitative differences alone distinguish stimuli triggering nontransformed T cell proliferation or death. J Immunol. 1992; 149(5):1583-1592. (Biology). View Reference
  11. Williams AF, Gagnon J. Neuronal cell Thy-1 glycoprotein: homology with immunoglobulin. Science. 1982; 216(4547):696-703. (Biology). View Reference
View All (11) View Less
553015 Rev. 14

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