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Purified Rat Anti-Mouse/Anti-Human IL-5
Purified Rat Anti-Mouse/Anti-Human IL-5
Human IL-5 ELISA Standard Curve. Purified TRFK5 antibody is used with the Biotin Rat Anti-Human IL-5 antibody (Cat. No. 554491) and Recombinant Human IL-5 (Cat. No. 554606) as the standard.
Human IL-5 ELISA Standard Curve. Purified TRFK5 antibody is used with the Biotin Rat Anti-Human IL-5 antibody (Cat. No. 554491) and Recombinant Human IL-5 (Cat. No. 554606) as the standard.
Product Details
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BD Pharmingen™
Mouse (QC Testing), Human (Tested in Development)
Rat IgG1, κ
Mouse Semi-Purified T-Cell Clone Supernatant
ELISA Capture (Routinely Tested), Intracellular block/flow cytometry, Neutralization (Tested During Development), Western blot (Reported)
0.5 mg/ml
Aqueous buffered solution containing ≤0.09% sodium azide.

Preparation And Storage

Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Recommended Assay Procedures

ELISA Capture: For a mouse IL-5 ELISA, purified TRFK5 antibody can be paired with the biotinylated TRFK4 anti-mouse IL-5 antibody (Cat. No. 554397) as the detecting antibody, with recombinant mouse IL-5 (Cat. No. 554581) as the standard. For a human IL-5 ELISA, purified TRFK5 antibody can be paired with the biotinylated JES1-5A10 anti-human IL-5 antibody (Cat. No. 554491) as the detecting antibody, with recombinant human IL-5 (Cat. No. 554606) as the standard. The purified TRFK5 antibody should be titrated 1.0 - 4.0 µg/ml to determine optimal concentration for ELISA capture. To obtain linear standard curves, doubling dilutions of IL-5 protein ranging ~2000 to 15 pg/ml are recommended for inclusion in each ELISA plate. For specific methodology, please visit the protocols section on our website at

Note: For testing IL-5 in serum or plasma, the Mouse IL-5 OptEIA™  Set (Cat. No. 555236) and the Human IL-5 OptEIA™  Set (Cat. No. 555202) are recommended.

Immunofluorescent Staining and Flow Cytometric Analysis: The TRFK5 antibody is useful for immunofluorescent staining and flow cytometric analysis to identify and enumerate mouse IL-5 producing cells within mixed cell populations.  PE- or APC-conjugated TRFK5 antibodies (Cat. No. 554395, Cat. No. 554396) are especially suitable for these experiments.

Neutralization: The BD Pharmingen™ NA/LE TRFK5 antibody (Cat. No. 554391) is useful for neutralization of mouse and human IL-5 bioactivity.

WB: The TRFK5 antibody has been found useful for Western blotting

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  3. Please refer to for technical protocols.
  4. Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use.
  5. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at
554393 Rev. 2
Antibody Details
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The TRFK5 antibody reacts with mouse interleukin-5 (IL-5) and cross-reacts with human IL-5. The TRFK5 antibody has been reported to cross react with IL-5 from rhesus monkey. This is a neutralizing antibody.

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Format Details
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Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
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Citations & References
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Development References (9)

  1. Abrams J. Immunoenzymetric assay of mouse and human cytokines using NIP-labeled anti-cytokine antibodies. Curr Protoc Immunol. 2001; 1:6.20-6.21. (Clone-specific: ELISA). View Reference
  2. Abrams JS, Roncarolo MG, Yssel H, Andersson U, Gleich GJ, Silver JE. Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples. Immunol Rev. 1992; 127:5-24. (Clone-specific: Neutralization). View Reference
  3. Assenmacher M, Schmitz J, Radbruch A. Flow cytometric determination of cytokines in activated murine T helper lymphocytes: expression of interleukin-10 in interferon-gamma and in interleukin-4-expressing cells. Eur J Immunol. 1994; 24(5):1097-1101. (Clone-specific: Flow cytometry). View Reference
  4. Mo XY, Sarawar SR, Doherty PC. Induction of cytokines in mice with parainfluenza pneumonia. J Virol. 1995; 69(2):1288-1291. (Clone-specific: ELISA). View Reference
  5. Sander B, Hoiden I, Andersson U, Moller E, Abrams JS. Similar frequencies and kinetics of cytokine producing cells in murine peripheral blood and spleen. Cytokine detection by immunoassay and intracellular immunostaining. J Immunol Methods. 1993; 166(2):201-214. (Clone-specific). View Reference
  6. Sarawar SR, Sangster M, Coffman RL, Doherty PC. Administration of anti-IFN-gamma antibody to beta 2-microglobulin-deficient mice delays influenza virus clearance but does not switch the response to a T helper cell 2 phenotype. J Immunol. 1994; 153(3):1246-1253. (Clone-specific: ELISA). View Reference
  7. Schumacher JH, O'Garra A, Shrader B, et al. The characterization of four monoclonal antibodies specific for mouse IL-5 and development of mouse and human IL-5 enzyme-linked immunosorbent. J Immunol. 1988; 141(5):1576-1581. (Clone-specific: Neutralization). View Reference
  8. Swain SL, Weinberg AD, English M, Huston G. IL-4 directs the development of Th2-like helper effectors. J Immunol. 1990; 145(11):3796-3806. (Clone-specific: Neutralization). View Reference
  9. Taguchi T, McGhee JR, Coffman RL, et al. Analysis of Th1 and Th2 cells in murine gut-associated tissues. Frequencies of CD4+ and CD8+ T cells that secrete IFN-gamma and IL-5. J Immunol. 1990; 145(1):68-77. (Clone-specific: ELISA). View Reference
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Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.