BD Pharmingen™ Purified NA/LE Rat Anti-Mouse IL-23 p19
Clone N71-1183 (RUO)
Flow cytometric analysis of Mouse IL-23 expression. 293-F cells, either transfected with mouse IL-23 p19 (solid line histograms) or untransfected (dotted line histograms), were fixed and permeabilized using BD Cytofix/Cytoperm™ Fixation/Permeabilization Kit (Cat. No. 554714) before staining with either Purified NA/LE Rat IgG1, κ Isotype Control (Cat. No. 553921) or NA/LE Rat Anti-Mouse IL-23 p19 (Cat. No. 565344). The second-step reagent was PE Goat Anti-Rat Ig (Cat. No. 550767). The histograms show the expression of IL-23 p19 (Right panel) or isotype control (Left panel) on gated events with the forward and side light-scatter characteristics of the intact cell population. Flow cytometry was performed on a BD LSRFortessa™ cell analyzer.
The N71-1183 monoclonal antibody limits the IL-23-induced production of IL-17 by MRL/lpr splenocytes. MRL/lpr splenocytes were activated in vitro in the presence of plate-bound Purified NA/LE Hamster Anti-Mouse CD3e (Cat. No. 553057) and Purified NA/LE Hamster Anti-Mouse CD28 (Cat. No. 553254) antibodies. Mouse IL-23 (10ng/ml) and either Purified NA/LE Rat IgG1, κ Isotype Control (Cat. No. 553921, left bar) or Purified NA/LE Rat Anti-Mouse IL-23 p19 (Cat. No. 565344, right bar) were added in the growth medium. After 24 hours, the concentrations of IL-17 in the supernatants were measured by ELISA. (Data provided by VC Kyttaris, Beth Israel Deaconess Medical Center.)
Flow cytometric analysis of Mouse IL-23 expression. 293-F cells, either transfected with mouse IL-23 p19 (solid line histograms) or untransfected (dotted line histograms), were fixed and permeabilized using BD Cytofix/Cytoperm™ Fixation/Permeabilization Kit (Cat. No. 554714) before staining with either Purified NA/LE Rat IgG1, κ Isotype Control (Cat. No. 553921) or NA/LE Rat Anti-Mouse IL-23 p19 (Cat. No. 565344). The second-step reagent was PE Goat Anti-Rat Ig (Cat. No. 550767). The histograms show the expression of IL-23 p19 (Right panel) or isotype control (Left panel) on gated events with the forward and side light-scatter characteristics of the intact cell population. Flow cytometry was performed on a BD LSRFortessa™ cell analyzer.
The N71-1183 monoclonal antibody limits the IL-23-induced production of IL-17 by MRL/lpr splenocytes. MRL/lpr splenocytes were activated in vitro in the presence of plate-bound Purified NA/LE Hamster Anti-Mouse CD3e (Cat. No. 553057) and Purified NA/LE Hamster Anti-Mouse CD28 (Cat. No. 553254) antibodies. Mouse IL-23 (10ng/ml) and either Purified NA/LE Rat IgG1, κ Isotype Control (Cat. No. 553921, left bar) or Purified NA/LE Rat Anti-Mouse IL-23 p19 (Cat. No. 565344, right bar) were added in the growth medium. After 24 hours, the concentrations of IL-17 in the supernatants were measured by ELISA. (Data provided by VC Kyttaris, Beth Israel Deaconess Medical Center.)
Preparation And Storage
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- An isotype control should be used at the same concentration as the antibody of interest.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Companion Products
The N71-1183 monoclonal antibody specifically recognizes the alpha subunit, also known as p19, of mouse interleukin 23 that is encoded by the Il23a gene. Interleukin 23 (IL-23) is a heterodimeric cytokine composed of a p40 subunit and a p19 subunit. The p19 subunit is unique to IL-23, while the p40 subunit is shared with IL-12 and is also known as IL-12B. Human and mouse p19 share 70% amino acid sequence identity. Dendritic cells and macrophages produce IL-23 in response to TLR2, TLR4 and TLR8 ligands, as well as the β-glucan receptor, Dectin-1. IL-23 has biological activities that are similar to, but distinct from IL-12: Both IL12 and IL-23 induce proliferation and IFN-γ production by human T cells. While IL-12 acts on both naïve and memory human T cells, the effects of IL-23 are restricted to memory T cells. IL-23, but not IL-12, has been shown to induce memory T cells to secret IL-17, a potent proinflammatory cytokine. In vitro and in vivo treatment with N71-1183 monoclonal antibody has been shown to inhibit IL-23's ability to enhance IL-17 production by activated MRL/lpr T lymphocytes (Kyttaris VC, Kampagianni O, Tsokos GC, 2013).
Development References (2)
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Kyttaris VC, Kampagianni O, Tsokos GC. Treatment with Anti-Interleukin 23 Antibody Ameliorates Disease in Lupus-Prone Mice.. Biomed Res Int. 2013; 2013Available: http://www.ncbi.nlm.nih.gov/pubmed/23841097 2014, December 3. View Reference
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Oppmann B, Lesley R, Blom B, et al. Novel p19 protein engages IL-12p40 to form a cytokine, IL-23, with biological activities similar as well as distinct from IL-12.. Immunity. 2000; 13(5):715-25. (Biology). View Reference
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