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      Purified NA/LE Rat Anti-Human IL-10
      Product Details
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      BD Pharmingen™
      Interleukin-10; CSIF; Cytokine synthesis inhibitory factor; TGIF
      Human (QC Testing), Viral (Tested in Development)
      Rat IgG2a, κ
      Recombinant Human IL-10
      ELISA (Routinely Tested), Intracellular staining (flow cytometry), Neutralization (Tested During Development)
      1.0 mg/ml
      AB_395519
      No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2µm sterile filtered. Endotoxin level is ≤0.01 EU/µg (≤0.001 ng/µg) of protein as determined by the LAL assay.
      RUO


      Preparation And Storage

      Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. This preparation contains no preservatives, thus it should be handled under aseptic conditions.
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      Recommended Assay Procedures

      ELISA: The Purified Rat Anti-Human IL-10 mAb (Clone JES3-19F1, Cat. No. 554705) has been reported to be useful as a capture antibody in sandwich ELISA for human IL-10. Purified JES3-19F1 antibody can be paired with the Biotin Anti-Human IL-10 mAb (Clone JES3-12G8, Cat. No. 554499) and with recombinant human IL-10 (Cat. No. 554611) as the standard. Purified JES3-19F1 antibody should be titrated to determine the optimal concentration for ELISA capture. To obtain linear standard curves, investigators may want to consider using doubling dilutions of human IL-10 standard from 15 pg/mL to 2000 pg/mL to be included in each ELISA plate. For measuring human IL-10 in complex biological fluids, such as serum or plasma, investigators are highly encouraged to use the BD OptEIA™ Human IL-10 ELISA Set (Cat. No. 555157) or BD OptEIA™ Human IL-10 ELISA Kit II (Cat. No. 550613) is recommended.

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      Product Notices

      1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      2. An isotype control should be used at the same concentration as the antibody of interest.
      3. Species cross-reactivity detected in product development may not have been confirmed on every format and/or application.
      4. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
      5. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
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      554703 Rev. 3
      Antibody Details
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      JES3-19F1

      The JES3-19F1 monoclonal antibody specifically recognizes human Interleukin-10 (IL-10) that is encoded by IL10.  IL-10 is also known as Cytokine Synthesis Inhibitory Factor (CSIF), B cell-derived T cell growth factor (B-TCGF), and T-cell growth inhibitory factor (TGIF). The JES3-19F1 antibody crossreacts with ebvIL-10 protein, the Epstein-Barr viral IL-10 homolog (viral IL-10 or vIL-10) encoded by the BCRF1 gene. IL-10 is produced by a variety of cells such as some activated T cells and B cells including regulatory T cells (Treg) and B cells (Breg), monocytes and macrophages, dendritic cells (DC), keratinocytes, and mast cells. IL-10 is a multifunctional cytokine that can downregulate immune and proinflammatory responses. IL-10 can act to reduce expression of major histocompatibility complex class II antigens, costimulatory molecules, or proinflammatory cytokines including IL-1β, IL-2, IL-3,  IL-12, IFN-γ, TNF or GM-CSF expressed by activated monocytes, macrophages, dendritic cells (DC), natural killer (NK) cells, or T cells. IL-10 has been shown to play a role in chronic viral infections. IL-10 can also enhance B cell survival, proliferation, and differentiation to become antibody-producing cells. The JES3-19F1 antibody reportedly neutralizes the biological activity of human IL-10 and ebvIL-10. IL-10 mediates its biological activities by signaling through a heterotetrameric receptor complex composed of the type II cytokine receptor subunits CD210a (IL-10 Rα) and CD210b (IL-10 Rβ).

      554703 Rev. 3
      Format Details
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      NA/LE
      NA/LE refers to the culture and purification methods and buffer used to produce purified antibodies with no azide and low endotoxin: Aqueous buffered solution containing no preservative, 0.2µm sterile filtered. Endotoxin level is ≤0.01 EU/µg (≤0.001 ng/µg) of protein as determined by the LAL assay.NA/LE are perfectly suited to be used in culture or in vivo (for nonhuman studies) for functional assays — blocking, neutralizing, activation or depletion — where the presence of azide may damage cells or exogenous endotoxin may signal or activate cells.
      NA/LE
      554703 Rev.3
      Citations & References
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      Development References (4)

      1. Andersson EC, Christensen JP, Marker O, Thomsen AR. Changes in cell adhesion molecule expression on T cells associated with systemic virus infection. Immunology. 1994; 152(3):1237-1245. (Biology). View Reference
      2. D'Andrea A, Aste-Amezaga M, Valiante NM, Ma X, Kubin M, Trinchieri G. Interleukin 10 (IL-10) inhibits human lymphocyte interferon gamma-production by suppressing natural killer cell stimulatory factor/IL-12 synthesis in accessory cells. J Exp Med. 1993; 178(3):1041-1048. (Biology). View Reference
      3. Prussin C, Metcalfe DD. Detection of intracytoplasmic cytokine using flow cytometry and directly conjugated anti-cytokine antibodies. J Immunol Methods. 1995; 188(1):117-128. (Methodology: Flow cytometry). View Reference
      4. Schlaak JF, Schmitt E, Hüls C, Meyer zum Büschenfelde KH, Fleischer B. A sensitive and specific bioassay for the detection of human interleukin-10. J Immunol Methods. 1994; 168(1):49-54. (Biology). View Reference
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      554703 Rev. 3

      Please refer to Support Documents for Quality Certificates


      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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