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PE Rat Anti-Mouse CD130
PE Rat Anti-Mouse CD130
Two-color flow cytometric analysis of CD130 expression on mouse splenocytes. BALB/c mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 (Mouse BD Fc Block™) (Cat. No. 553141 or 553142). The cells were then stained with APC Hamster Anti-Mouse CD3e (Cat. No. 553066 or 561826) and either PE Rat IgG2a, κ Isotype Control (Cat.No. 553930; Left Panel) or PE Rat Anti-Mouse CD130 antibody (Cat. No. 566726; Right Panel) at 0.5ug/test. The pseudocolor dot plots showing CD130 (or Ig Isotype control) staining versus CD3e were derived from gated events with the forward and side light-scatter characteristics of viable splenocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa™ X-20 Cell Analyzer System and FlowJo™ software. Data shown on this Technical Data Sheet are not lot specific.
Two-color flow cytometric analysis of CD130 expression on mouse splenocytes. BALB/c mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 (Mouse BD Fc Block™) (Cat. No. 553141 or 553142). The cells were then stained with APC Hamster Anti-Mouse CD3e (Cat. No. 553066 or 561826) and either PE Rat IgG2a, κ Isotype Control (Cat.No. 553930; Left Panel) or PE Rat Anti-Mouse CD130 antibody (Cat. No. 566726; Right Panel) at 0.5ug/test. The pseudocolor dot plots showing CD130 (or Ig Isotype control) staining versus CD3e were derived from gated events with the forward and side light-scatter characteristics of viable splenocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa™ X-20 Cell Analyzer System and FlowJo™ software. Data shown on this Technical Data Sheet are not lot specific.
Product Details
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BD Pharmingen™
IL-6 receptor subunit beta; IL-6R-beta; IL-6RB; Il6st; gp130; membrane glycoprotein 130; oncostatin-M receptor subunit alpha
Mouse (QC Testing)
Rat WI, also known as Wistar (outbred) IgG2a, κ
Mouse Extracellular CD130 Recombinant Protein
Flow cytometry (Routinely Tested)
0.2 mg/ml
AB_2739772
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. An isotype control should be used at the same concentration as the antibody of interest.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  5. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
566726 Rev. 1
Antibody Details
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V49-2176

The V49-2176 monoclonal antibody specifically binds to CD130, which is also known as gp130 (Membrane glycoprotein 130) and IL-6RB/IL-6Rβ (Interleukin-6 Receptor beta), that is encoded by the Il6st (Interleukin-6 signal transducer) gene. CD130 is a 130-kDa type 1 transmembrane glycoprotein that serves as a common signal-transducing receptor for several class I cytokine receptor (also known as hematopoietin receptor) complexes. Cytokine receptor complexes that contain gp130 mediate the biological effects of several pleiotropic cytokines, including IL-6, IL-11, IL-27, Cardiotrophin-1 (CT-1), Ciliary neurotrophic factor (CNTF), Leukemia inhibitory factor (LIF), and Oncostatin M (OSM). CD130 is widely expressed in adult and embryonic tissues including T cells, activated B cells, monocytes, plasma cells, and endothelia.

566726 Rev. 1
Format Details
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PE
R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
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PE
Yellow-Green 488 nm, 532 nm, 561 nm
496 nm, 566 nm
576 nm
566726 Rev.1
Citations & References
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Development References (4)

  1. Saito M, Yoshida K, Hibi M, Taga T, Kishimoto T.. Molecular cloning of a murine IL-6 receptor-associated signal transducer, gp130, and its regulated expression in vivo.. J Immunol.. 1992; 148(12):4066-71. (Biology). View Reference
  2. Silver JS, Hunter CA.. gp130 at the nexus of inflammation, autoimmunity, and . J Leukoc Biol. 2010; 88(6):1145-56. (Biology). View Reference
  3. Wang X, Lupardus P, Laporte SL, Garcia KC.. Structural biology of shared cytokine receptors.. Annu Rev Immunol.. 2009; 27:29-60. (Biology). View Reference
  4. Wilkinson AN, Gartlan KH, Kelly G, et al. Granulocytes Are Unresponsive to IL-6 Due to an Absence of gp130. J Immunol.. 2018. Available: http://www.jimmunol.org/content/jimmunol/early/2018/04/05/jimmunol.1701191.full.pdf April 25, 2018. View Reference
View All (4) View Less
566726 Rev. 1

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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.