Two-color flow cytometric analysis of TCR Vβ3 expression on human peripheral blood T lymphocytes. Human peripheral blood cells were stained with BD Horizon™ BV421 Mouse Anti-Human CD3 antibody (Cat. No. 562426/562427) and either PE Mouse IgG2a, κ Isotype Control (Cat.No. 554648; Left Plot) or PE Mouse Anti-Human TCR Vβ3 antibody (Cat. No. 566432; Right Plot). Erythrocytes were lysed with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899). Two-color flow cytometric contour plots showing the correlated expression of TCR Vβ3 (or Ig Isotype control staining) versus CD3 were derived from gated events with the forward and side light-scatter characteristics of viable human lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa™ X-20 Flow Cytometer System.
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Two-color flow cytometric analysis of TCR Vβ3 expression on human peripheral blood T lymphocytes. Human peripheral blood cells were stained with BD Horizon™ BV421 Mouse Anti-Human CD3 antibody (Cat. No. 562426/562427) and either PE Mouse IgG2a, κ Isotype Control (Cat.No. 554648; Left Plot) or PE Mouse Anti-Human TCR Vβ3 antibody (Cat. No. 566432; Right Plot). Erythrocytes were lysed with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899). Two-color flow cytometric contour plots showing the correlated expression of TCR Vβ3 (or Ig Isotype control staining) versus CD3 were derived from gated events with the forward and side light-scatter characteristics of viable human lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa™ X-20 Flow Cytometer System.
Product Details
BD Pharmingen™
TCR V beta 3; TCR Vb3; TCR BV3; TRBV3-1
Human (QC Testing)
Mouse IgG2a, κ
Human TCR Vβ3-Cβ1 TCR
Flow cytometry (Routinely Tested)
5 µl
AB_2739729
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
RUO
Preparation And Storage
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.
Product Notices
- This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
- An isotype control should be used at the same concentration as the antibody of interest.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Companion Products
Stain Buffer (FBS) RUO
Size
500 mL
Cat No.
554656
Stain Buffer (BSA) RUO
Size
500 mL
Cat No.
554657
Lysing Buffer RUO
Size
100 mL
Cat No.
555899
Lysing Solution 10X Concentrate IVD
Size
100 mL
Cat No.
349202
PE Mouse IgG2a, κ Isotype Control RUO
Size
0.1 mg
Cat No.
554648
BV421 Mouse Anti-Human CD3 RUO
Size
100 Tests
Cat No.
562426
566432 Rev. 1
Antibody Details
JOVI.3
The JOVI.3 monoclonal antibody specifically recognizes the human variable beta 3 region of the T cell receptor for antigen (TCR Vβ3). The JOVI.3 antibody reportedly stains approximately 1-10% of human CD3-positive peripheral blood lymphocytes including CD4+ or CD8+ T cells. The variation in human TCR Vβ3 expression correlates with allelic polymorphism in the spacer region of recombination signal sequence. The JOVI.3 antibody is mitogenic for peripheral blood T cells expressing TCR Vβ3.
566432 Rev. 1
Format Details
PE
R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
PE
Yellow-Green 488 nm, 532 nm, 561 nm
496 nm, 566 nm
576 nm
566432 Rev.1
Citations & References
Development References (3)
-
Moss P, Gillespie G, Frodsham P, Bell J, Reyburn H. Clonal populations of CD4+ and CD8+ T cells in patients with multiple myeloma and paraproteinemia. Blood. 1996; 87(8):3296-3306. (Clone-specific: Flow cytometry). View Reference
-
Posnett DN, Vissinga CS, Pambuccian C, et al. Level of human TCRBV3S1 (V beta 3) expression correlates with allelic polymorphism in the spacer region of the recombination signal sequence. J Exp Med. 1994; 179(5):1707-1711. (Biology). View Reference
-
Viney JL, Prosser HM, Hewitt CR, Lamb JR, Owen MJ. Generation of monoclonal antibodies against a human T cell receptor beta chain expressed in transgenic mice. Hybridoma. 1992; 11(6):701-713. (Immunogen: Flow cytometry, Functional assay, Immunoprecipitation, Radioimmunoassay). View Reference
566432 Rev. 1
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
