Skip to main content Skip to navigation
PE Mouse Anti-Human CD99
PE Mouse Anti-Human CD99
Profile of peripheral blood lymphocytes analyzed on a FACScan (BDIS, San Jose, CA)
PE Mouse Anti-Human CD99
Multiparameter flow cytometric analysis of CD99 expression on human peripheral blood leucocytes populations. Whole blood was stained with either PE Mouse IgG2a, κ Isotype Control (Cat. No. 555574; Left Plot) or PE Mouse Anti-Human CD99 antibody (Cat. No. 555689; Right Plot). Erythrocytes were lysed with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899). Bivariate pseudocolor density plots showing CD99 expression (or Ig Isotype control staining) versus side light-scatter (SSC-A) signals were derived from gated events with the forward and side light-scattering characteristics of intact leucocyte populations. Flow cytometry and data analysis were performed using a BD LSRFortessa™ Cell Analyzer System and FlowJo™ software.
Profile of peripheral blood lymphocytes analyzed on a FACScan (BDIS, San Jose, CA)
Multiparameter flow cytometric analysis of CD99 expression on human peripheral blood leucocytes populations. Whole blood was stained with either PE Mouse IgG2a, κ Isotype Control (Cat. No. 555574; Left Plot) or PE Mouse Anti-Human CD99 antibody (Cat. No. 555689; Right Plot). Erythrocytes were lysed with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899). Bivariate pseudocolor density plots showing CD99 expression (or Ig Isotype control staining) versus side light-scatter (SSC-A) signals were derived from gated events with the forward and side light-scattering characteristics of intact leucocyte populations. Flow cytometry and data analysis were performed using a BD LSRFortessa™ Cell Analyzer System and FlowJo™ software.
Product Details
Down Arrow Up Arrow


BD Pharmingen™
E2; MIC2
Human (QC Testing)
Mouse IgG2a, κ
Flow cytometry (Routinely Tested)
20 µl
IV N92
4267
AB_396040
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The antibody was conjugated to the dye under optimum conditions and unconjugated antibody and free dye were removed.

Recommended Assay Procedures

BD® CompBeads can be used as surrogates to assess fluorescence spillover (Compensation). When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells.  However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.

Product Notices

  1. This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
  2. An isotype control should be used at the same concentration as the antibody of interest.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  5. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  6. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  7. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
555689 Rev. 7
Antibody Details
Down Arrow Up Arrow
TÜ12

The TÜ12 monoclonal antibody specifically recognizes CD99, also referred to as E2 antigen, a 32 kDa sialoglycoprotein expressed on all leucocyte lineages. The E2 antigen is the MIC2 gene product and is differentially expressed during T- and B-lymphoid and granulocytic development, with higher densities being expressed during early hematopoietic stages. Mature granulocytes express  very little or no CD99. E2 has been shown to be involved in T-cell adhesion processes and is suggested to have a functional role in hematopoietic adhesion pathways.

This antibody is routinely tested by flow cytometric analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.

555689 Rev. 7
Format Details
Down Arrow Up Arrow
PE
R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
altImg
PE
Yellow-Green 488 nm, 532 nm, 561 nm
496 nm, 566 nm
576 nm
555689 Rev.7
Citations & References
Down Arrow Up Arrow

Development References (6)

  1. Aussel C, Bernard G, Breittmayer JP, Pelassy C, Zoccola D, Bernard A. Monoclonal antibodies directed against the E2 protein (MIC2 gene product) induce exposure of phosphatidylserine at the thymocyte cell surface. Biochemistry. 1993; 32(38):10096-10101. (Biology). View Reference
  2. Chang A, Benda PM, Wood BL, Kussick SJ. Lineage-specific identification of nonhematopoietic neoplasms by flow cytometry.. Am J Clin Pathol. 2003; 119(5):643-55. (Clone-specific: Flow cytometry). View Reference
  3. Dworzak MN, Fritsch G, Buchinger P, et al. Flow cytometric assessment of human MIC2 expression in bone marrow, thymus, and peripheral blood. Blood. 1994; 83(2):415-425. (Biology). View Reference
  4. Gelin C, Aubrit F, Phalipon A, et al. The E2 antigen, a 32 kd glycoprotein involved in T-cell adhesion processes, is the MIC2 gene product. EMBO J. 1989; 8(11):3253-3259. (Biology). View Reference
  5. Knapp W. W. Knapp .. et al., ed. Leucocyte typing IV : white cell differentiation antigens. Oxford New York: Oxford University Press; 1989:1-1182.
  6. Uchańska-Ziegler B, Wernet P, Ziegler A. Differentiation of a human myeloid cell line (HL-60) toward granulocyte- and macrophage-like cells: comparison of cell surface antigen expression.. Haematol Blood Transfus. 1983; 28:386-8. (Clone-specific: Immunofluorescence). View Reference
View All (6) View Less
555689 Rev. 7

Please refer to Support Documents for Quality Certificates


Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.