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      PE Mouse Anti-Human CD179b (λ5)
      PE Mouse Anti-Human CD179b (λ5)
      Flow cytometric analysis of CD179b expression on human NALM6 cells. Cells from the human NALM6 (ATCC CRL-3273) cell line were preincubated with Human BD Fc Block™ (Cat. No. 564219/564220). Cells were stained with either PE Mouse IgG1, κ Isotype Control (Cat No. 554680, dashed line histogram) or PE Mouse Anti-Human CD179b (Cat No. 568078; solid line histogram) at 0.5 ug/test. The fluorescence histogram showing CD179b expression (or Ig Isotype control staining) was derived from gated events with the forward and side light-scatter characteristics of intact NALM6 cells. Flow cytometric analysis was performed using a BD LSRFortessa™ Flow Cytometer System and FlowJo™ software. Data shown on this Technical Data Sheet are not lot specific.
      PE Mouse Anti-Human CD179b (λ5)
      Flow cytometric analysis of CD179b expression on human NALM6 cells. Cells from the human NALM6 (ATCC CRL-3273) cell line were preincubated with Human BD Fc Block™ (Cat. No. 564219/564220). Cells were stained with either PE Mouse IgG1, κ Isotype Control (Cat No. 554680, dashed line histogram) or PE Mouse Anti-Human CD179b (Cat No. 568078; solid line histogram) at 0.5 ug/test. The fluorescence histogram showing CD179b expression (or Ig Isotype control staining) was derived from gated events with the forward and side light-scatter characteristics of intact NALM6 cells. Flow cytometric analysis was performed using a BD LSRFortessa™ Flow Cytometer System and FlowJo™ software. Data shown on this Technical Data Sheet are not lot specific.
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      BD Pharmingen™
      IGL1; IGLL1; VPREB2; ig lambda-5; immunoglobulin omega polypeptide chain; immunoglobulin-related 14.1 protein; lambda5
      Human (QC Testing)
      Mouse BALB/c IgG1, κ
      Ltk– preBCR transfectants
      Flow cytometry (Routinely Tested)
      0.2 mg/ml
      VII
      Aqueous buffered solution containing ≤0.09% sodium azide.
      RUO


      Preparation And Storage

      Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions and unconjugated antibody and free dye were removed.
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      Recommended Assay Procedures

      BD® CompBeads can be used as surrogates to assess fluorescence spillover (Compensation). When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and BD CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.

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      Product Notices

      1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      2. An isotype control should be used at the same concentration as the antibody of interest.
      3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      4. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
      5. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
      6. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
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      568078 Rev. 1
      Antibody Details
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      HSL11.rMAb

      The HSL11.rMAb monoclonal antibody specifically recognizes CD179b, which is also known as Ig Lambda 5, λ5 or Ig omega. CD179b is a ~23 kDa protein within the immunoglobulin gene superfamily and is a component of the pre-B cell receptor complex (pre-BCR). The pre-BCR is a heterodimer comprised of an immunoglobulin heavy chain (IgH) and a surrogate light (SL) chain, which is composed of non-covalently linked CD179b (λ5) and CD179a (VpreB) proteins. The pre-BCR is expressed during the early stages of B lymphocytes development and its expression is restricted to pre-B cell stage of B cell development, whereas the expression of SL chain is restricted to both pro-B and pre-B cell stages of B cell development. Due to restricted lineage expression, CD179b (λ5) along with CD179a (VpreB) may serve as a useful marker of precursor B cells and can be utilized for immunophenotyping precursor B cell lymphoblastic lymphomas. The pre-BCR plays a role in signal transduction for cell proliferation and differentiation from the proB cell to the preB cell stage, allelic exclusion of IgH chain loci, and Ig light chain gene rearrangements. CD179b (λ5) is required for precursor B cell development and its deficiency could prevent the maturation and survival of the B cell precursors beyond the pre-B cell stage and can lead to B cell deficiency and agammaglobulinemia.

      568078 Rev. 1
      Format Details
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      PE
      R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
      altImg
      PE
      Yellow-Green 488 nm, 532 nm, 561 nm
      496 nm, 566 nm
      576 nm
      568078 Rev.1
      Citations & References
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      Development References (3)

      1. Kiyokawa N, Sekino T, Matsui T, et al. Diagnostic importance of CD179a/b as markers of precursor B-cell lymphoblastic lymphoma.. Mod Pathol. 2004; 17(4):423-9. (Clone-specific). View Reference
      2. Tsuganezawa K, Kiyokawa N, Matsuo Y, et al. Flow cytometric diagnosis of the cell lineage and developmental stage of acute lymphoblastic leukemia by novel monoclonal antibodies specific to human pre-B-cell receptor.. Blood. 1998; 92(11):4317-24. (Immunogen). View Reference
      3. Winkler TH, Mårtensson IL. The Role of the Pre-B Cell Receptor in B Cell Development, Repertoire Selection, and Tolerance. Front Immunol. 2018; 15(9):2423. (Biology). View Reference
      568078 Rev. 1

      Please refer to Support Documents for Quality Certificates


      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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