The M293 monoclonal antibody specifically recognizes the integrin ß7 chain (also known as integrin ßp), which is found in association with either of two α chains. α4ß7 (LPAM-1) is expressed on most mature lymphocytes and on small subsets of thymic and bone marrow cells; it interacts with several ligands, including VCAM-1, fibronectin, and MAdCAM-1. αIELß7 is found primarily on intestinal mucosal and intraepithelial lymphocytes (IEL), and it may be primarily involved in interactions between IEL and epithelia via recognition of E-cadherin. The epitope recognized by the mAb M293 has been mapped to the region between amino acids 387 and 542. The M293 antibody exhibits little or no activity in blocking ß7 integrin-mediated adhesion.
The antibody was conjugated to BD Horizon™ BV480 which is part of the BD Horizon Brilliant™ Violet family of dyes. With an Ex Max of 436-nm and Em Max at 478-nm, BD Horizon BV480 can be excited by the violet laser and detected in the BD Horizon BV510 (525/40-nm) filter set. BV480 has less spillover into the BV605 detector and, in general, is brighter than BV510.