CD85 molecules belong to a large immunoregulatory family and it has been clustered into different subclasses from CD85a to CD85m in the VIIth HLDA workshop. CD85j is also called as Ig-like transcript (ILT2), or leukocyte Ig-like receptor (LIR-1). Reacts with an 110 kDa membrane glycoprotein expressed on a subset of NK cells, which varies amongst individuals, and a subpopulation of T lymphocytes. Expression on T lymphocytes, NK cells may depend on the individuals tested. Function studies show that ligation of ILT2 with MHC class I including HLA-A, B, G1 and -E induces an inhibitory signal via recruitment of SHP-1 phosphatase.
The antibody was conjugated to BD Horizon™ BV480 which is part of the BD Horizon Brilliant™ Violet family of dyes. With an Ex Max of 436-nm and Em Max at 478-nm, BD Horizon BV480 can be excited by the violet laser and detected in the BD Horizon BV510 (525/40-nm) filter set. BV480 has less spillover into the BV605 detector and, in general, is brighter than BV510.