The 9B9 monoclonal antibody specifically binds to CD368, which is also known as CLEC6 (C-type lectin-like receptor 6), CLEC4D, CLECSF8, and MCL (macrophage C-type lectin). CD368 is a 30 kDa type II transmembrane glycoprotein that belongs to the C-type Lectin Receptor family of molecules. It is primarily expressed on peripheral blood neutrophils and monocytes and weakly on several subsets of blood dendritic cells. Expression of this receptor is lost upon in vitro differentiation of monocytes into dendritic cells or macrophages. CD368 reportedly serves as an endocytic receptor that may function in antigen clearance and presentation to T lymphocytes. CD368 may also play roles in cellular adhesion, signaling and glycoprotein turnover. CD368 expression can be upregulated by cells in response to cytokines including IFN-γ, TNF, IL-6, and IL-10.
The antibody was conjugated to BD Horizon™ BV480 which is part of the BD Horizon Brilliant™ Violet family of dyes. With an Ex Max of 436-nm and Em Max at 478-nm, BD Horizon BV480 can be excited by the violet laser and detected in the BD Horizon BV510 (525/40-nm) filter set. BV480 has less spillover into the BV605 detector and, in general, is brighter than BV510.