BUV737 Mouse Anti-Human CD127
Multicolor flow cytometric analysis of CD127 expression on human peripheral blood CD4+ T lymphocytes. Human whole blood was stained with APC Mouse Anti-Human CD4 (Cat. No. 561841/555349/561840) and PE Mouse Anti-Human CD25 (Cat. No. 555432/557138/560989) antibodies, and either BD Horizon™ BUV737 Mouse IgG1, κ Isotype Control (Cat. No. 612758; Left Plot) or BD Horizon BUV737 Mouse Anti-Human CD127 antibody (Cat. No. 612794/612795; Right Plot). Erythrocytes were lysed with BD FACS™ Lysing Solution (Cat. No. 349202). The pseudocolor density plot showing the correlated expression of CD25 (or Ig Isotype control staining) versus CD127 was derived from CD4+ gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa™ Cell Analyzer System and FlowJo™ software.
BD Horizon™ BUV737 Mouse Anti-Human CD127
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