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BB700 Mouse Anti-Human CD220
Product Details
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BD OptiBuild™
Insulin Receptor; Insulin R; IR; INSR; HHF5
Human (Tested in Development)
Mouse IgG1, κ
Flow cytometry (Qualified)
0.2 mg/ml
Aqueous buffered solution containing ≤0.09% sodium azide.

Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon BB700 under optimal conditions that minimize unconjugated dye and antibody.

Recommended Assay Procedures

For optimal and reproducible results, BD Horizon Brilliant Stain Buffer should be used anytime two or more BD Horizon Brilliant dyes are used in the same experiment. Fluorescent dye interactions may cause staining artifacts which may affect data interpretation. The BD Horizon Brilliant Stain Buffer was designed to minimize these interactions. More information can be found in the Technical Data Sheet of the BD Horizon Brilliant Stain Buffer (Cat. No. 563794 or 566349).

When setting up compensation, it is recommended to compare spillover values obtained from cells and BD™ CompBeads to ensure that beads will provide sufficiently accurate spillover values.

For optimal results, it is recommended to perform two washes after staining with antibodies. Cells may be prepared, stained with antibodies and washed twice with wash buffer per established protocols for immunofluorescent staining prior to acquisition on a flow cytometer. Performing fewer than the recommended wash steps may lead to increased spread of the negative population.

Product Notices

  1. This antibody was developed for use in flow cytometry.
  2. The production process underwent stringent testing and validation to assure that it generates a high-quality conjugate with consistent performance and specific binding activity. However, verification testing has not been performed on all conjugate lots.
  3. Researchers should determine the optimal concentration of this reagent for their individual applications.
  4. An isotype control should be used at the same concentration as the antibody of interest.
  5. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  6. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at
  7. Please refer to for technical protocols.
  8. BD Horizon Brilliant Stain Buffer is covered by one or more of the following US patents: 8,110,673; 8,158,444; 8,575,303; 8,354,239.
  9. BD Horizon Brilliant Blue 700 is covered by one or more of the following US patents: 8,455,613 and 8,575,303.
  10. Cy is a trademark of GE Healthcare.
746083 Rev. 1
Antibody Details
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The 3B6 monoclonal antibody specifically recognizes the Insulin Receptor (IR), a glycoprotein composed of two extracellular α-subunits (130 kDa) and two transmembrane β-subunits (95 kDa). It is expressed on human hematopoietic and non-hematopoietic cells, but unlike the insulin-like growth factor receptor (IGF-IR), which is ubiquitous, the insulin receptor is restricted to major target tissues of insulin action. Upon binding insulin, the IR subunits form a heterotetramer of two α and two β subunits resulting in autophosphorylation and activation of the tyrosine kinase activity of the receptor. This ligand-receptor interaction is important in regulating cell metabolism and growth. 3B6/IR monoclonal antibody reacts similarly to anti-human IR α, clone 47-9, an α-subunit antibody.

The antibody was conjugated to BD Horizon™ BB700, which is part of the BD Horizon Brilliant™ Blue family of dyes.   It is a polymer-based tandem dye developed exclusively by BD Biosciences.  With an excitation max of 485 nm and an emission max of 693 nm, BD Horizon BB700 can be excited by the 488 nm laser and detected in a standard PerCP-Cy™5.5 set (eg, 695/40-nm filter). This dye provides a much brighter alternative to PerCP-Cy5.5 with less cross laser excitation off the 405 nm and 355 nm lasers.

746083 Rev. 1
Format Details
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The BD Horizon Brilliant™ Blue 700 (BB700) Dye is part of the BD Horizon Brilliant™ Blue family of dyes. This tandem fluorochrome is comprised of a polymer-technology dye donor with an excitation maximum (Ex Max) of 476-nm and an acceptor dye with an emission maximum (Em Max) at 695-nm. Driven by BD innovation, BB700 is designed to be excited by the blue laser (488-nm) and detected using an optical filter centered near 695-nm (e.g., a 695/20-nm bandpass filter). The donor dye can be excited by the Violet (405 nm) laser and the acceptor dye can be excited by the red (627–640 nm) laser resulting in cross-laser excitation and fluorescence spillover. BB700 Reagents are significantly brighter than equivalent PerCP or PerCP-Cy5.5 reagents and are less sensitive to photobleaching. In addition, BB700 shows much less excitation by the violet (407-nm) laser resulting in less spillover. BB700 has minimal yellow green (562-nm) excitation and is ideal for instruments with both blue (488-nm) and yellow green (562-nm) lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
Blue 488 nm
476 nm
695 nm
746083 Rev.1
Citations & References
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Development References (7)

  1. Brindle NP, Tavare JM, Dickens M, Whittaker J, Siddle K. Anti-(insulin receptor) monoclonal antibody-stimulated tyrosine phosphorylation in cells transfected with human insulin receptor cDNA. Biochem J. 1990; 268(3):615-620. (Biology). View Reference
  2. Hagino H, Shii K, Yokono K, et al. Enzyme-linked immunosorbent assay method for human autophosphorylated insulin receptor. Applicability to insulin-resistant states.. Diabetes. 1994; 43(2):274-80. (Clone-specific: ELISA). View Reference
  3. Okada Y, Yoshida M, Baba S, Shii K. Development of vanadate sensitive human erythrocytes insulin receptor tyrosine phosphatase assay.. Diabetes Res Clin Pract. 1998; 41(3):157-63. (Clone-specific). View Reference
  4. Prigent SA, Stanley KK, Siddle K. Identification of epitopes on the human insulin receptor reacting with rabbit polyclonal antisera and mouse monoclonal antibodies. J Biol Chem. 1990; 265(17):9970-9979. (Biology). View Reference
  5. Shima F, Ishida Y, Hotta K, et al. Autophosphorylation of insulin receptor in a patient with Werner's syndrome associated with insulin resistant diabetes mellitus.. Endocr J. 1995; 42(1):107-13. (Clone-specific: ELISA). View Reference
  6. Soos MA, O'Brien RM, Brindle NP, et al. Monoclonal antibodies to the insulin receptor mimic metabolic effects of insulin but do not stimulate receptor autophosphorylation in transfected NIH 3T3 fibroblasts.. Proc Natl Acad Sci USA. 1989; 86(14):5217-21. (Biology). View Reference
  7. Soos MA, Siddle K, Baron MD, et al. Monoclonal antibodies reacting with multiple epitopes on the human insulin receptor.. Biochem J. 1986; 235(1):199-208. (Biology). View Reference
View All (7) View Less
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Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.