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APC Mouse Anti-Human CD122
APC Mouse Anti-Human CD122
Two-color flow cytometric analysis of CD122 expression on human peripheral blood leucocytes. Human whole blood was stained with PE Mouse Anti-Human CD56 antibody (Cat. No. 555516/561903) and either APC Mouse IgG1, κ Isotype Control (Cat. No. 554681; Left Plot) or APC Mouse Anti-Human CD122 antibody (Cat. No. 566620; Right Plot). Erythrocytes were lysed with BD FACS Lysing Solution (Cat. No. 349202). Two-color flow cytometric dot plots showing the correlated expression of CD122 (or Ig Isotype control staining) versus CD56 were derived from gated events with the forward and side light-scatter characteristics of intact leucocyte populations. Flow cytometric analysis was performed using a BD LSRFortessa™ Cell Analyzer System.
Two-color flow cytometric analysis of CD122 expression on human peripheral blood leucocytes. Human whole blood was stained with PE Mouse Anti-Human CD56 antibody (Cat. No. 555516/561903) and either APC Mouse IgG1, κ Isotype Control (Cat. No. 554681; Left Plot) or APC Mouse Anti-Human CD122 antibody (Cat. No. 566620; Right Plot). Erythrocytes were lysed with BD FACS Lysing Solution (Cat. No. 349202). Two-color flow cytometric dot plots showing the correlated expression of CD122 (or Ig Isotype control staining) versus CD56 were derived from gated events with the forward and side light-scatter characteristics of intact leucocyte populations. Flow cytometric analysis was performed using a BD LSRFortessa™ Cell Analyzer System.
Product Details
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BD Pharmingen™
IL2RB; IL-2Rβ; IL-2R subunit beta; IL-2RB; IL15RB; IL-2/15Rb; IL-2R p75
Human (QC Testing)
Mouse BALB/c IgG1, κ
Human YTS Cell Line
Flow cytometry (Routinely Tested)
5 µl
V C046
3560
AB_2869796
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to APC under optimum conditions, and unconjugated antibody and free APC were removed.

Product Notices

  1. This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
  2. An isotype control should be used at the same concentration as the antibody of interest.
  3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  5. This APC-conjugated reagent can be used in any flow cytometer equipped with a dye, HeNe, or red diode laser.
  6. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  7. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
566620 Rev. 1
Antibody Details
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Mik-β3

The Mik-β3 monoclonal antibody specifically binds to CD122. CD122 is also known as IL-2/15Rβ because it is the shared p75 subunit (IL-12Rβ/IL-15Rβ) of heteromeric receptors for IL-2 and IL-15. CD122 is a 70-75 kD type I transmembrane glycoprotein expressed on  thymocytes, T cells, B cells, NK cells, monocytes, hematopoietic progenitor cells, fetal liver cells and stromal cells. The CD122 plays roles in the activation, differentiation and proliferation of various cell types including T cells, B cells and NK cells.

566620 Rev. 1
Format Details
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APC
Allophycocyanin (APC), is part of the BD family of phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 651 nm and an emission maximum (Em Max) at 660 nm. APC is designed to be excited by the Red (627-640 nm) laser and detected using an optical filter centered near 660 nm (e.g., a 660/20 nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
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APC
Red 627-640 nm
651 nm
660 nm
566620 Rev.1
Citations & References
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Development References (5)

  1. Ishikawa T, Uchiyama T, Kamio M, Onishi R, Kodaka T, Okuma M. IL-4 down-regulates IL-2 receptor p75 by accelerating its endocytosis. Int Immunol. 1991; 3(6):517-525. (Biology). View Reference
  2. Kikutani H, Kishimoto T. The cytokine receptors: section report. In: Schlossman SF. Stuart F. Schlossman .. et al., ed. Leucocyte typing V : white cell differentiation antigens : proceedings of the fifth international workshop and conference held in Boston, USA, 3-7 November, 1993. Oxford: Oxford University Press; 1995:1855-1864.
  3. Schlossman SF. Stuart F. Schlossman .. et al., ed. Leucocyte typing V : white cell differentiation antigens : proceedings of the fifth international workshop and conference held in Boston, USA, 3-7 November, 1993. Oxford: Oxford University Press; 1995.
  4. Tsudo M, Kitamura F, Miyasaka M. Characterization of the interleukin 2 receptor beta chain using three distinct monoclonal antibodies. Proc Natl Acad Sci U S A. 1989; 86(6):1982-1986. (Immunogen: Immunoprecipitation). View Reference
  5. Voss SD, Robb RJ, Weil-Hillman G, et al. Increased expression of the interleukin 2 (IL-2) receptor beta chain (p70) on CD56+ natural killer cells after in vivo IL-2 therapy: p70 expression does not alone predict the level of intermediate affinity IL-2 binding. J Exp Med. 1990; 172(4):1101-1114. (Clone-specific: Flow cytometry). View Reference
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566620 Rev. 1

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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.