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      PE Mouse Anti-Human CD62P
      Product Details
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      BD™
      SELP; PSEL; P-selectin; PADGEM; LECAM3; GMP-140; GRMP; GMP140
      Human
      Mouse BALB/c IgG1, κ
      Activated Human Platelets
      Flow cytometry
      1.5 μg/mL
      20 μL
      V S058
      6403
      Phosphate buffered saline with gelatin and 0.1% sodium azide.
      RUO (GMP)


      Preparation And Storage

      The monoclonal antibody is supplied as 3 µg purified immunoglobulin in 2.0 mL (1.5 µg/mL) of phosphate-buffered saline (PBS). The PE conjugate is supplied as 3 µg in 2.0 mL (1.5 µg/mL) of PBS. PBS contains gelatin and 0.1% sodium azide. Vials should be stored at 2° to 8°C. Conjugated forms should Not be frozen and should be protected from prolonged exposure to light. Each reagent is stable for the period shown on the bottle label when stored as directed.

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      348107 Rev. 1
      Antibody Details
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      AC1.2

      The CD62P antibody, clone AC1.2, is derived from the hybridization of NS-1 mouse myeloma cells with spleen cells isolated from BALB/c mice immunized with activated platelets.

      The CD62P antibody recognizes a molecule known as platelet activation-dependent granule-external membrane (PADGEM) protein or granule membrane protein (GMP-140), which is a 140-kilodalton (kDa) single-chain polypeptide. The CD62P antigen is a member of the selectin family of adhesion molecules and mediates the adhesion of activated platelets to neutrophils and monocytes in hemostasis.

      348107 Rev. 1
      Format Details
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      PE
      R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
      altImg
      PE
      Yellow-Green 488 nm, 532 nm, 561 nm
      496 nm, 566 nm
      576 nm
      348107 Rev.1
      Citations & References
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      View product citations for antibody "348107" on CiteAb

      Development References (11)

      1. Abrams CS, Ellison N, Budzynski AZ, Shattil SJ. Direct detection of activated platelets and platelet-derived microparticles in humans.. Blood. 1990; 75(1):128-38. (Biology). View Reference
      2. Ault KA, Rinder HM, Mitchell JG, Rinder CS, Lambrew CT, Hillman RS. Correlated measurement of platelet release and aggregation in whole blood. Cytometry. 1989; 10:448-455. (Biology).
      3. Berman CL, Yeo EL, Wencel-Drake JD, Furie BC, Ginsberg MH, Furie B. A platelet α-granule membrane protein that is associated with the plasma membrane after activation. J Clin Invest. 1986; 78:130-137. (Biology).
      4. Bonfanti R, Furie BC, Furie B, Wagner DD. PADGEM (GMP-140) is a component of Weibel-Palade bodies of human endothelial cells. Blood. 1989; 73:1109-1112. (Biology).
      5. Diacovo T, Springer TA. CD62L (L-selectin) cluster report. In: Schlossman SF. Stuart F. Schlossman .. et al., ed. Leucocyte typing V : white cell differentiation antigens : proceedings of the fifth international workshop and conference held in Boston, USA, 3-7 November, 1993. Oxford: Oxford University Press; 1995:1503-1504.
      6. Hamburger SA, McEver RP. GMP-140 mediates adhesion of stimulated platelets to neutrophils. Blood. 1990; 75:550-554. (Biology).
      7. Larsen E, Celi A, Gilbert GE, et al. PADGEM protein: a receptor that mediates the interaction of activated platelets with neutrophils and monocytes.. Cell. 1989; 59(2):305-12. (Biology). View Reference
      8. Metzelaar MJ, Sixma JJ, Nieuwenhuis HK. Detection of platelet activation using activation-specific monoclonal antibodies. Blood Cells. 1990; 16:85-96. (Biology).
      9. Modderman PW. Knapp W, Dörken B, Gilks WR, et al, ed. Leucocyte Typing IV: White Cell Differentiation Antigens. New York, NY: Oxford University Press; 1989:1038-1039.
      10. Parmentier S, Kaplan C, Catimel B, McGregor JL. New families of adhesion molecules play a vital role in platelet functions.. Immunol Today. 1990; 11(7):225-7. (Biology). View Reference
      11. Stenberg PE, McEver RP, Shuman MA, Jacques YV, Bainton DF. A platelet α-granule membrane protein (GMP-140) is expressed on the plasma membrane after activation. J Cell Biol. 1985; 101:880-886. (Biology).
      View All (11) View Less
      348107 Rev. 1

      Please refer to Support Documents for Quality Certificates


      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures. 

       

      Although not required, these products are manufactured in accordance with Good Manufacturing Practices.