V450 Mouse Anti-Human CD21

BD Horizon™ V450 Mouse Anti-Human CD21

Name: V450 Mouse Anti-Human CD21
Brand: BD Horizon™
SKU: 658169

Clone B-ly4

(RUO (GMP))

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Product Details

Brand: BD Horizon™

Alternative Name: CR2; Complement receptor type 2; C3DR; EBV-R; Epstein-Barr virus receptor

Reactivity: Human

Isotype: Mouse IgG1, κ

Application: Flow cytometry

Concentration: 13 µg/mL

Vol. Per Test: 5 μL

Workshop Number: IV B98

Entrez Gene ID: 1380

Regulatory Status: RUO (GMP)

658169 Rev. 1

Antibody Details

B-ly4

The CD21 antibody, clone B-ly4, is derived from the hybridization of mouse myeloma cells with spleen cells from immunized mice.

The CD21 antibody specifically binds to a 145-kilodalton (kDa) glycosylated type I integral membrane protein. The CD21 antigen is also known as complement receptor type 2 (CR2), C3d receptor, and Epstein-Barr virus receptor (EBV-R).

658169 Rev. 1

Format Details

V450

BD Horizon™ V450 Dye is part of the BD Horizon™ violet family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 405-nm and an emission maximum (Em Max) at 450-nm. BD Horizon™ V450, driven by BD innovation, is designed to be excited by the violet laser (405 nm) and detected using an optical filter centered near 450-nm (e.g., a 450/50-nm bandpass filter). The dye can be excited by the UV (355-nm) laser resulting in cross-laser excitation and spillover. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.

Format: V450

Excitation Source: Violet 405 nm

Excitation Max: 405 nm

Emission Max: 450 nm

658169 Rev.1

Citations & References

Development References (8)

Boyd AW, Fecondo J. Role of the CD21 antigen in lymphocyte transformation by Epstein-Barr virus. Immunol Cell Biol. 1988; 66:159-165. (Biology).
Cherukuri A, Shoham T, Sohn HW, et al. The tetraspanin CD81 is necessary for partitioning of coligated CD19/CD21-B cell antigen receptor complexes into signaling-active lipid rafts. J Immunol. 2004; 172:370-380. (Biology).
Dörken B, Möller P, Pezzutto A, Schwartz-Albiez R, Moldenhauer G. Knapp W, Dörken B, Gilks WR, et al, ed. Leucocyte Typing IV: White Cell Differentiation Antigens. New York, NY: Oxford University Press; 1989:34-36.
Fearon DT, Carroll MC. Regulation of B lymphocyte responses to foreign and self-antigens by the CD19/CD21 complex. Annu Rev Immunol. 2000; 18:393-422. (Biology).
Mongini PK, Jackson AE, Tolani S, Fattah RJ, Inman JK. Role of complement-binding CD21/CD19/CD81 in enhancing human B cell protection from Fas-mediated apoptosis. J Immunol. 2003; 171:5244-5254. (Biology).
Weis JJ, Tedder TF, Fearon DT. Identification of a 145,000 Mr membrane protein as the C3d receptor (CR2) of human B lymphocytes. Proc Natl Acad Sci USA. 1984; 81:881-885. (Biology).
Zola H, Swart B, Nicholson I, Voss E. Leukocyte and Stromal Cell Molecules: The CD Markers. 2007. (Biology).
van Dongen JJ, Lhermitte L, Böttcher S, et al. EuroFlow antibody panels for standardized n-dimensional flow cytometric immunophenotyping of normal, reactive and malignant leukocytes. Leukemia. 2012; 26(9):1908-1975. (Biology). View Reference

View All (8)

658169 Rev. 1

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