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TCRαβ FITC/TCRγδ PE/CD3 PerCP-Cy™5.5

TCRαβ FITC/TCRγδ PE/CD3 PerCP-Cy™5.5

(CE_IVD)
Product Details
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BD Oncomark™
Human
Flow cytometry
CE_IVD
Phosphate buffered saline with BSA and 0.1% sodium azide.


Description

BD Oncomark™ Anti–TCR-α/β-1 FITC/Anti–TCR-γ/δ-1 PE/CD3 PerCP-Cy5.5 is intended for in vitro flow cytometric immunophenotyping of hematologic disorders.


BD Oncomark™ Anti–TCR-α/β-1 FITC/Anti–TCR-γ/δ-1 PE/CD3 PerCP-Cy5.5 is intended for in vitro flow cytometric immunophenotyping of hematologic disorders.

Preparation And Storage

The antibody reagent is stable until the expiration date shown on the label when stored at 2° to 8°C. Do not use after the expiration date. Do not freeze the reagent or expose it to direct light during storage or incubation with cells. Keep the outside of the reagent vial dry.

Do not use the reagent if you observe any change in appearance. Precipitation or discoloration indicates instability or deterioration.

335836 Rev. 1
Components
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Description Clone Isotype
gamma delta TCR PE 11F2 IgG1,
CD3 PerCP-Cy5.5 SK7 IgG1, κ
TCR αβ FITC WT31 IgG1, κ
335836 Rev. 1
Citations & References
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Development References (18)

  1. Borst J, van Dongen JJ, Bolhuis RL, et al. Distinct molecular forms of human T cell receptor gamma/delta detected on viable T cells by a monoclonal antibody.. J Exp Med. 1988; 167(5):1625-44. (Biology). View Reference
  2. Brenner MB, Groh V, Porcelli SA, et al. Knapp W, Dörken B, Gilks WR, et al, ed. Leucocyte Typing IV: White Cell Differentiation Antigens. 1986:145-149.
  3. Brenner MB, Groh V, Porcelli SA, et al. Knapp W, Dörken B, Gilks WR, et al, ed. Leucocyte Typing IV: White Cell Differentiation Antigens. 1986:145-149.
  4. Centers for Disease Control. Update: universal precautions for prevention of transmission of human immunodeficiency virus, hepatitis B virus, and other bloodborne pathogens in healthcare settings. MMWR. 1988; 37:377-388. (Biology).
  5. Clevers H, Alarcón B, Wileman T, Terhorst C. The T cell receptor/CD3 complex: a dynamic protein ensemble. Annual Rev Immunol. 1988; 6:629. (Biology).
  6. Consensus protocol for the flow cytometric immunophenotyping of hematopoietic malignancies. Rothe G, Schmitz G. Leukemia. 1996; 10:877-895. (Biology).
  7. Lanier LL, Ruitenberg J, Bolhuis RL, Borst J, Phillips JH, Testi R. Structural and serological heterogeneity of gamma/delta T cell antigen receptor expression in thymus and peripheral blood.. Eur J Immunol. 1988; 18(12):1985-92. (Biology). View Reference
  8. Lanier LL, Serafini AT, Ruitenberg JJ, et al. The γ T-cell antigen receptor. J Clin Immunol. 1987; 7:429-440. (Biology).
  9. Lanier LL, Weiss A. Presence of Ti (WT31) negative T lymphocytes in peripheral blood and thymus. Nature. 1986; 324:268. (Biology).
  10. NCCLS document. 2001. (Biology).
  11. Oettgen HC, Kappler J, Tax WJ, Terhorst C. Characterization of the two heavy chains of the T3 complex on the surface of human T lymphocytes. J Biol Chem. 1984; 259(19):12039-12048. (Biology). View Reference
  12. Reichert T, DeBruyere M, Deneys V, et al. Lymphocyte subset reference ranges in adult Caucasians. Clin Immunol Immunopathol. 1991; 60(2):190-208. (Biology). View Reference
  13. Slameron A, Sanchez-Madrid F, Ursa MA, Fresno M, and Alarcon B. A conformational epitope expressed upon association of the CD3-epsilon with either CD3-delta or CD3-gamma is the main target for recognition by anti-CD3 monoclonal antibodies. J Immunol. 1991; 147:3047-3052. (Biology).
  14. Spits H, Borst J, Tax W, Capel PJ, Terhorst C, de Vries JE. Characteristics of a monoclonal antibody (WT-31) that recognizes a common epitope on the human T cell receptor for antigen.. J Immunol. 1985; 135(3):1922-8. (Biology). View Reference
  15. Stelzer GT, Marti G, Hurley A, McCoy PJ, Lovett EJ, Schwartz A. US-Canadian consensus recommendations on the immunophenotypic analysis of hematologic neoplasia by flow cytometry: standardization and validation of laboratory procedures. Cytometry. 1997; 30:214-230. (Biology).
  16. Testi R, Lanier LL. Functional expression of CD28 on T cell antigen receptor γ/δ-bearing T lymphocytes. Eur J Immunol. 1989; 19:185-188. (Biology).
  17. Weiss A, Newton M, Crommie D. Expression of T3 in association with a molecule distinct from the T-cell antigen receptor heterodimer.. Proc Natl Acad Sci USA. 1986; 83(18):6998-7002. (Biology). View Reference
  18. van Dongen JJM, Krissansen GW, Wolvers-Tettero ILM, et al. Cytoplasmic expression of the CD3 antigen as a diagnostic marker for immature T-cell malignancies. Blood. 1988; 71:603-612. (Biology).
View All (18) View Less
335836 Rev. 1

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For In Vitro Diagnostic Use.

 

23-22942-00