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CD3 FITC/CD16+CD56 PE/CD45 PerCP with BD Trucount™ Tubes

CD3 FITC/CD16+CD56 PE/CD45 PerCP with BD Trucount™ Tubes

(CE_IVD)
Product Details
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BD Tritest™
Human
Flow cytometry
CE_IVD
Buffered saline with BSA and 0.1% sodium azide.


Description

BD Tritest™ CD3 fluorescein isothiocyanate (FITC)/CD16 + CD56 phycoerythrin (PE)/CD45 peridinin chlorophyll protein (PerCP) is a threecolor direct immunofluorescence reagent for use with a suitably equipped flow cytometer to identify and determine the percentages and absolute counts of mature human T lymphocytes (CD3+) and natural killer (NK) (CD3–CD16+CD56+) lymphocyte subsets in erythrocyte-lysed whole blood. When used with BD Trucount™ tubes, absolute counts of these populations can be enumerated from a single tube. BD Tritest reagent and BD Trucount tubes can be used with the BD FACS™ Loader. The reagent can be used with or without an isotype control.


BD Tritest™ CD3 fluorescein isothiocyanate (FITC)/CD16 + CD56 phycoerythrin (PE)/CD45 peridinin chlorophyll protein (PerCP) is a threecolor direct immunofluorescence reagent for use with a suitably equipped flow cytometer to identify and determine the percentages and absolute counts of mature human T lymphocytes (CD3+) and natural killer (NK) (CD3–CD16+CD56+) lymphocyte subsets in erythrocyte-lysed whole blood. When used with BD Trucount™ tubes, absolute counts of these populations can be enumerated from a single tube. BD Tritest reagent and BD Trucount tubes can be used with the BD FACS™ Loader. The reagent can be used with or without an isotype control.

Preparation And Storage

1. Store the reagent at 2–8°C. Do not use after the expiration date shown on the label.

2. Do not freeze the reagent or expose it to direct light during storage or incubation with cells. Keep the reagent vial dry.

3. Store TruCOUNT Tubes in their original foil pouch at 2–25°C. To avoid potential condensation, open the pouch only after ithas reached room temperature and carefully reseal the pouch immediately after removing a tube. Examine the desiccant each time you open the pouch. If the desiccant has turned from blue to lavender, discard the remaining tubes. Use tubes within 1 hour after removal from the foil pouch and do not use beyond the expiration date indicated on the packaging.

342442 Rev. 1
Components
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Description Clone Isotype
CD16 (FcγRIII) PE B73.1 IgG1, κ
CD56 (NCAM-1) PE NCAM16.2 IgG2b, κ
CD3 FITC SK7 IgG1, κ
CD45 PerCP C8Mab-1 IgG2b, λ
342442 Rev. 1
Citations & References
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Development References (30)

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  15. Lanier LL, Le AM, Civin CI, Loken MR, Phillips JH. The relationship of CD16 (Leu-11) and Leu-19 (NKH-1) antigen expression on human peripheral blood NK cells and cytotoxic T lymphocytes. J Immunol. 1986; 136(12):4480-4486. (Biology). View Reference
  16. Nicholson J, Browning S, Orloff S, McDougal J. Inactivation of HIV-infected H9 cells in whole blood preparations by lysing/fixing reagents used in flow cytometry. J Immunol Methods. 1993; 160:215-218. (Biology).
  17. Nicholson J, Kidd P, Mandy F, Livnat D, Kagan J. Use of CD45 fluorescence and side-scatter characteristics for gating lymphocytes when using the whole blood lysis procedure and flow cytometry. Cytometry. 1996; 26:227-230. (Biology).
  18. Nicholson JKA, Hubbard M, Jones BM. Use of CD45 fluorescence and side-scatter characteristics for gating lymphocytes when using the whole blood lysis procedure and flow cytometry. Cytometry. 1996; 26:16-21. (Biology).
  19. Nicholson JKA, Jones BM, Hubbard M. CD4 T-lymphocyte determinations on whole blood specimens using a single-tube three-color assay. Cytometry. 1993; 14:685-689. (Biology).
  20. Perussia B, Acuto O, Terhorst C, et al. Human natural killer cells analyzed by B73-1, a monoclonal antibody blocking Fc receptor functions, II: studies of B73-1 antibody-antigen interaction on the lymphocyte membrane. J Immunol. 1983; 130:2142-2148. (Biology).
  21. Perussia B, Starr S, Abraham S, Fanning V, Trinchieri G. Human natural killer cells analyzed by B73.1, a monoclonal antibody blocking Fc receptor functions. I. Characterization of the lymphocyte subset reactive with B73.1. J Immunol. 1983; 130(5):2133-2141. (Biology). View Reference
  22. Perussia B, Trinchieri G, Jackson A, et al. The Fc receptor for IgG on human natural killer cells: phenotypic, functional, and comparative studies with monoclonal antibodies. J Immunol. 1984; 133(1):180-189. (Biology). View Reference
  23. Prince HE, Hirji K, Waldbeser LS, Plaeger-Marshall S, Kleinman S, Lanier LL. Influence of racial background on the distribution of T-cell subsets and Leu 11-positive lymphocytes in healthy blood donors. Diagn Immunol. 1985; 3(1):33-37. (Biology).
  24. Procedures for the Collection of Diagnostic Blood Specimens by Venipuncture: Approved Standard. NCCLS document H3-A3. 1991. (Biology).
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  27. Schmidt RE. Non-lineage/natural killer section report: new and previously defined clusters. In: Knapp W. W. Knapp .. et al., ed. Leucocyte typing IV : white cell differentiation antigens. Oxford New York: Oxford University Press; 1989:517-542.
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  30. van Dongen JJM, Krissansen GW, Wolvers-Tettero ILM, et al. Cytoplasmic expression of the CD3 antigen as a diagnostic marker for immature T-cell malignancies. Blood. 1988; 71:603-612. (Biology).
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342442 Rev. 1

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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For In Vitro Diagnostic Use.

 

23-22942-00