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CD103 FITC/CD22 PE/CD20 PerCP-Cy™5.5

CD103 FITC/CD22 PE/CD20 PerCP-Cy™5.5

(CE_IVD)
Product Details
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BD Oncomark™
Human
Flow cytometry
CE_IVD
Phosphate buffered saline with BSA and 0.1% sodium azide.


Description

BD Oncomark™ CD103 FITC/CD22 PE/CD20 PerCP-Cy5.5 is intended for in vitro flow cytometric immunophenotyping of normal and abnormal subsets of B lymphocytes. The reagents detect expression of the CD103, CD22, and CD20 antigens. These antigens are highly expressed in the abnormal cells of hairy-cell leukemia. A subset of normal B cells expressing CD103 has been identified in peripheral blood. CD103/CD22/CD20 assays are used in the diagnosis of hematologic disorders.


BD Oncomark™ CD103 FITC/CD22 PE/CD20 PerCP-Cy5.5 is intended for in vitro flow cytometric immunophenotyping of normal and abnormal subsets of B lymphocytes. The reagents detect expression of the CD103, CD22, and CD20 antigens. These antigens are highly expressed in the abnormal cells of hairy-cell leukemia. A subset of normal B cells expressing CD103 has been identified in peripheral blood. CD103/CD22/CD20 assays are used in the diagnosis of hematologic disorders.

Preparation And Storage

The antibody reagent is stable until the expiration date shown on the label when stored at 2° to 8°C. Do not use after the expiration date. Do not freeze the reagent or expose it to direct light during storage or incubation with cells. Keep the outside of the reagent vial dry.

Do not use the reagent if you observe any change in appearance. Precipitation or discoloration indicates instability or deterioration.

333174 Rev. 1
Components
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Description Clone Isotype
CD22 PE S-HCL-1 IgG2b, κ
CD20 PerCP-Cy™5.5 14D4 IgG2a,
CD103 FITC Ber-ACT8 IgG1, κ
333174 Rev. 1
Citations & References
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Development References (23)

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  8. Hultin LE, Hausner MA, Hultin PM, Giorgi JV. CD20 (pan-B cell) antigen is expressed at a low level on asubpopulation of human T lymphocytes. Cytometry. 1993; 14:196-204. (Biology).
  9. Jackson AL, Warner NL. Rose NR, Friedman H, Fahey JL, ed. Manual of Clincial Laboratory Immunology, Third Edition. Washington DC: American Society for Microbiology; 1986:226-235.
  10. Kruschwitz M, Fritzsche G, Schwarting R, et al. Ber-ACT8: new monoclonal antibody to the mucosa lymphocyte antigen. J Clin Pathol. 1991; 44(8):636-645. (Biology). View Reference
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  15. Micklem KJ, Dong Y, Willis A, et al. HML-1 antigen on mucosa-associated T cells, activated cells, and hairy leukemic cells is a new integrin containing the β7 subunit. Am J Pathol. 1996; 3:30-36. (Biology).
  16. NCCLS document. 2001. (Biology).
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  18. Parker CM, Cepek KL, Russell GJ, et al. A family of beta 7 integrins on human mucosal lymphocytes. Proc Natl Acad Sci U S A. 1992; 89(5):1924-1928. (Biology). View Reference
  19. Schwarting R, Stein H, Wang CY. The monoclonal antibodies alpha S-HCL 1 (alpha Leu-14) and alpha S-HCL 3 (alpha Leu-M5) allow the diagnosis of hairy cell leukemia. Blood. 1985; 65(4):974-983. (Biology). View Reference
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333174 Rev. 1

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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For In Vitro Diagnostic Use.

 

23-22942-00