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Purified Mouse Anti-Human XPA
Purified Mouse Anti-Human XPA

Western blot analysis of XPA. A Jurkat cell lysate (Human T-cell leukemia; ATCC TIB-152) was probed with the mouse anti-human XPA antibody (clone 12F5) at a concentration of 1 µg/ml (lane 1), 0.5 µg/ml (lane 2), or 0.25 µg/ml (lane 3).

Western blot analysis of XPA. A Jurkat cell lysate (Human T-cell leukemia; ATCC TIB-152) was probed with the mouse anti-human XPA antibody (clone 12F5) at a concentration of 1 µg/ml (lane 1), 0.5 µg/ml (lane 2), or 0.25 µg/ml (lane 3).

Product Details
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BD Pharmingen™
Xeroderma Pigmentosum factor A
Human (QC Testing)
Mouse IgG2a, κ
Human XPA (His-tagged) Recombinant Protein
Western blot (Routinely Tested), Immunoprecipitation (Tested During Development)
34-40 kDa
0.5 mg/ml
AB_396423
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C.

Recommended Assay Procedures

Recommended Assay Procedure:

Western blot:  Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
556453 Rev. 8
Antibody Details
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12F5

Nucleotide excision repair (NER) is a major pathway by which cells remove UV and chemically induced damage from DNA. The biochemistry of NER is complex and includes recognition of the damaged DNA, formation of incisions ~26-29 nucleotides apart on each side of the damaged DNA, excision of an oligonucleotide carrying the damaged DNA, and synthesis of a repair patch using the opposite DNA strand as a template. The xeroderma pigmentosum (XP) factors are the best characterized components in the NER pathway. They are termed XP-A to -G and are thought to be required for the first steps of the nucleotide excision repair process. XPA is a DNA damage-binding protein and XPC is a single stranded DNA-binding protein. XPB and XPD are DNA helicases that are components of the transcription factor TFIIH. The TFIIH complex is thought to be involved in transcription and NER. XPF is an endonuclease that binds to ERCC1 (for excision repair cross-complementing) and the ERCC1-XPF complex makes the incision 5' to the DNA damage. ERCC1 migrates at a molecular weight of ~36 kDa in SDS-PAGE. XPG is an endonuclease that makes the 3' incision. XPA has been reported to migrate at a molecular weight of 34-40 kDa in SDS-PAGE.

556453 Rev. 8
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
556453 Rev.8
Citations & References
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Development References (2)

  1. Aboussekhra A, Biggerstaff M, Shivji MK, et al. Mammalian DNA nucleotide excision repair reconstituted with purified protein components. Cell. 1995; 80(6):859-868. (Biology). View Reference
  2. Evans E, Fellows J, Coffer A, Wood RD. Open complex formation around a lesion during nucleotide excision repair provides a structure for cleavage by human XPG protein. EMBO J. 1997; 16(3):625-638. (Biology). View Reference
556453 Rev. 8

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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.