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Purified Mouse Anti-Vti1b
Purified Mouse Anti-Vti1b

Western blot analysis of Vti1b on a human endothelial cell lysate.  Lane 1: 1:1000, lane 2: 1:2000, lane 3: 1:4000 dilution of the mouse anti-Vti1b antibody.

Purified Mouse Anti-Vti1b

Immunofluorescence staining of NIH/3T3 cells (Mouse embryo fibroblast cells; ATCC CRL-1658).

Western blot analysis of Vti1b on a human endothelial cell lysate.  Lane 1: 1:1000, lane 2: 1:2000, lane 3: 1:4000 dilution of the mouse anti-Vti1b antibody.

Immunofluorescence staining of NIH/3T3 cells (Mouse embryo fibroblast cells; ATCC CRL-1658).

Product Details
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BD Transduction Laboratories™
Human (QC Testing), Mouse, Rat (Tested in Development)
Mouse IgG1
Mouse Vti1b aa. 9-121
Western blot (Routinely Tested), Immunofluorescence (Tested During Development)
27 kDa
250 µg/ml
AB_398927
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.

Recommended Assay Procedures

Western blot:  Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
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Antibody Details
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7/Vti1b

Eukaryotic protein trafficking involves packaging of target molecules into membranous vesicles that bud from a donor compartment, travel to a specific destination, fuse, and release their contents into an acceptor compartment. Recognition between vesicle and acceptor membrane is mediated by the pairing of the integral membrane SNARE proteins. The stable interaction between vesicle proteins (v-SNAREs; VAMP1, VAMP2) and target proteins (t-SNAREs;  syntaxin 1, SNAP-25) juxtaposes the membranes and results in an activated docked state and/or membrane fusion. With the identification of all SNARE family members in yeast, the research focus has turned to mammalian cells. Here, sequence analysis has identified additional SNARE proteins, including VTI1a and VTI1b. In line with their involvement in vesicle transport, these molecules are expressed in a wide range of mammalian tissues. VTI1b is a membrane bound protein whose localization overlaps with the cis/medial Golgi marker mannosidase II and the trans-Golgi marker syntaxin 6. VTI1b interacts with, and disrupts the localization of, syntaxin 5. Thus, VTI1b is thought to function in the regulation of post-Golgi vesicle trafficking.

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Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
611405 Rev.1
Citations & References
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Development References (5)

  1. Advani RJ, Bae HR, Bock JB, et al. Seven novel mammalian SNARE proteins localize to distinct membrane compartments. J Biol Chem. 1998; 273(17):10317-10324. (Biology). View Reference
  2. Chen K, Manga P, Orlow SJ. Pink-eyed dilution protein controls the processing of tyrosinase. Mol Cell Biol. 2002; 13(6):1953-1964. (Biology: Immunofluorescence). View Reference
  3. Mallard F, Tang BL, Galli T. Early/recycling endosomes-to-TGN transport involves two SNARE complexes and a Rab6 isoform. J Cell Biol. 2002; 156(4):653-664. (Biology: Western blot). View Reference
  4. Shorter J, Beard MB, Seemann J, Dirac-Svejstrup AB, Warren G. Sequential tethering of Golgins and catalysis of SNAREpin assembly by the vesicle-tethering protein p115. J Cell Biol. 2002; 157(1):45-62. (Biology: Western blot). View Reference
  5. Yang B, Gonzalez L, Prekeris R, Steegmaier M, Advani RJ, Scheller RH. SNARE interactions are not selective. Implications for membrane fusion specificity. J Biol Chem. 1999; 274(9):5649-5653. (Biology). View Reference
View All (5) View Less
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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.