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Purified Mouse Anti-La Protein
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Product Details
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BD Transduction Laboratories™
Human (QC Testing), Rat, Dog, Rabbit (Tested in Development)
Mouse IgG1
Human La Protein aa. 179-289
Western blot (Routinely Tested), Immunofluorescence (Tested During Development)
47 kDa
250 µg/ml
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.

Recommended Assay Procedures

Western blot: Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml .

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
610904 Rev. 1
Antibody Details
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44/La Protein

La was first found to bind autoantibodies from individuals with Sjogren's syndrome and systemic lupus erythematosus (SLE). La is a transcription factor for RNA polymerase III (Pol III). It participates in the termination of Pol III-mediated transcription and the recycling of the transcriptional machinery. La associates with the 3' oligo U portion of transcripts generated by Pol III. In some cases, this association protects the message from 3' processing. The association of La and mRNA is mediated by the presence of an N-terminal ribonucleoprotein (RNP) consensus sequence in La. The La protein also binds several small RNAs, such as 7S RNA, 5S RNA, and tRNA, and is thought to be involved in their biogenesis. It is known that histone mRNA is stabilized throughout S Phase and is degraded only at the end of S phase. La protein participates in the stabilization of histone mRNA which results in increased production of histone protein. These data suggest that a major function of La is to protect vital nascent transcripts from premature degradation.

610904 Rev. 1
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
610904 Rev.1
Citations & References
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Development References (3)

  1. Chambers JC, Kenan D, Martin BJ, Keene JD. Genomic structure and amino acid sequence domains of the human La autoantigen. J Biol Chem. 1988; 263(34):18043-18051. (Biology). View Reference
  2. Goodier JL, Fan H, Maraia RJ. A carboxy-terminal basic region controls RNA polymerase III transcription factor activity of human La protein. Mol Cell Biol. 1997; 17(10):5823-5832. (Biology). View Reference
  3. McLaren RS, Caruccio N, Ross J. Human La protein: a stabilizer of histone mRNA. Mol Cell Biol. 1997; 17(6):3028-3036. (Biology). View Reference
610904 Rev. 1

Please refer to Support Documents for Quality Certificates


Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.