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Purified Mouse Anti-JNK/SAPK1
Product Details
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BD Transduction Laboratories™
p49 MAPK
Rat (QC Testing), Human, Mouse, Dog, Chicken, Frog (Tested in Development)
Mouse IgG1
Human MAPKp49 aa. 264-415
Western blot (Routinely Tested), Immunofluorescence, Immunohistochemistry (Tested During Development), Immunoprecipitation (Not Recommended)
49kDa
250 µg/ml
AB_397959
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.

Recommended Assay Procedures

Western blot: Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml .

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
610628 Rev. 1
Antibody Details
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37/pan-JNK/SAPK1

The Ras signaling pathway links the signals from growth factor receptors with the activation of the MAPK kinase cascade of phosphorylation leading to cell growth and differentiation. External stimuli, such as endotoxins, UV irradiation, heat, and hyperosmolarity, induce an array of cellular responses that culminate with gene expression, ultimately dictating an adaptation to the new environment. The ultimate cellular response to environmental stress is cell death or apoptosis. This pathway is regulated by Rac1 and Cdc42 which link Jun Kinases (JNKs), such as p38. JNK is a c-Jun kinase that was also identified as SAPK1 and MAPKp49. JNK contains homology with the MAP/ERK family of kinases, and contains an N-terminal ATP-binding site and two autophosphorylation "TXY" sites. JNKK1/MEKK4/SEK1 is a dual-specificity kinase activated by conditions of cellular stress, such as heat shock and hyperosmolarity. JNKK1 phosphorylates JNK and p38 but not the ERK MAP kinases. Active JNK and p38 phosphorylate multiple transcription factors that induce expression of genes, such as proinflammatory cytokines.

610628 Rev. 1
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
610628 Rev.1
Citations & References
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Development References (2)

  1. Hinton DR, Henderson VW, Blanks JC, Rudnicka M, Miller CA. Monoclonal antibodies react with neuronal subpopulations in the human nervous system. J Comp Neurol. 1988; 267(3):398-408. (Biology). View Reference
  2. Mohit AA, Martin JH, Miller CA. p493F12 kinase: a novel MAP kinase expressed in a subset of neurons in the human nervous system. Neuron. 2001; 14(1):76-78. (Biology). View Reference
610628 Rev. 1

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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.