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Purified Mouse Anti-JAB1/CSN5
Product Details
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BD Transduction Laboratories™
Human (QC Testing), Mouse, Rat (Tested in Development)
Mouse IgG1
Human JAB1 aa. 234-334
Western blot (Routinely Tested), Immunofluorescence (Tested During Development)
38 kDa
250 µg/ml
AB_399061
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.

Recommended Assay Procedures

Western blot: Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
611618 Rev. 1
Antibody Details
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42/JAB1

JAB1 (Jun activation domain-binding protein-1) was isolated in a yeast two- hybrid screen using the c-Jun N-terminal activation domain. Binding of JAB1 to Jun potentiates activator protein transcription factor (AP-1) target gene transcription. JAB1 interacts with c-Jun and JunD, but not with JunB or v-Jun. It also interacts with other transcriptional regulatory complexes, such as the activator of stromelysin 1 gene transcription complex (RNF4/SPBP) and the steroid receptor coactivator-1 complex. Additionally, JAB1 is the CSN5 subunit of the COP9 signalosome, which contains multiple proteins with homologies to proteins present in the 19S subunit of the proteosome. Interestingly, JAB1 has been implicated in the instigation of p27kip1 and rat lutropin/choriogonadotropin receptor degradation. JAB1 has also been found to be involved in integrin signaling cascades, because it colocalizes with the integrin LFA-1 receptor and is translocated to the nucleus, where it enhances transactivation of the AP-1- dependent promoter following integrin stimulation. Thus, JAB1 is a multifunctional protein that regulates both gene transcription and protein degradation.  

611618 Rev. 1
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
611618 Rev.1
Citations & References
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Development References (5)

  1. Chauchereau A, Georgiakaki M, Perrin-Wolff M, Milgrom E, Loosfelt H. JAB1 interacts with both the progesterone receptor and SRC-1. J Biol Chem. 2000; 275(12):8540-8548. (Biology). View Reference
  2. Claret FX, Hibi M, Dhut S, Toda T, Karin M. A new group of conserved coactivators that increase the specificity of AP-1 transcription factors. Nature. 1996; 383(6599):453-457. (Biology). View Reference
  3. Ishida N, Hara T, Kamura T, Yoshida M, Nakayama K, Nakayama KI. Phosphorylation of p27Kip1 on serine 10 is required for its binding to CRM1 and nuclear export. J Biol Chem. 2002; 277(17):14355-14358. (Clone-specific: Western blot). View Reference
  4. Li S, Liu X, Ascoli M. p38JAB1 binds to the intracellular precursor of the lutropin/choriogonadotropin receptor and promotes its degradation. J Biol Chem. 2000; 275(18):13386-13393. (Biology). View Reference
  5. Tomoda K, Kubota Y, Kato J. Degradation of the cyclin-dependent-kinase inhibitor p27Kip1 is instigated by Jab1. Nature. 1999; 398(6723):160-165. (Biology). View Reference
View All (5) View Less
611618 Rev. 1

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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.