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Purified Mouse Anti-Human Na+, K+ ATPase β3
Purified Mouse Anti-Human Na+, K+ ATPase β3

Western blot analysis of Na+,K+ ATPase β3 on a human endothelial cell lysate. Lane 1: 0.25 µg/mL, lane 2: 0.125 µg/mL, lane 3: 0.0625 µg/mL of the mouse anti-human Na+, K+ ATPase β3 antibody.

Purified Mouse Anti-Human Na+, K+ ATPase β3

Immunofluorescence staining of HeLa cells (Human cervical epitheloid carcinoma; ATCC CCL-2).

Western blot analysis of Na+,K+ ATPase β3 on a human endothelial cell lysate. Lane 1: 0.25 µg/mL, lane 2: 0.125 µg/mL, lane 3: 0.0625 µg/mL of the mouse anti-human Na+, K+ ATPase β3 antibody.

Immunofluorescence staining of HeLa cells (Human cervical epitheloid carcinoma; ATCC CCL-2).

Product Details
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BD Transduction Laboratories™
Human (QC Testing)
Mouse IgG1
Human Na+, K+ ATPase β3 aa. 124-243
Western blot (Routinely Tested), Immunofluorescence (Tested During Development)
42 kDa
250 µg/ml
AB_398306
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at -20°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Recommended Assay Procedures

Western blot:  Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
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Antibody Details
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46/Na+,K+ ATPase β3

Na+,K+-ATPase is an integral plasma membrane protein that maintains the intracellular concentrations of Na+ and K+ ions. It is a P class pump that is composed of two α and two β transmembrane subunits. It is also thought to contain a γ subunit. The 120 kDa α subunit represents the catalytic site. Although the 50 kDa β subunit mediates proper folding of newly synthesized α subunits, it is not directly involved in ion transfer. There are four known α isoforms (α1, α2, α3, and α4) and three β isoforms (β1, β2, and β3). The Na+,K+-ATPase is ubiquitously expressed in the plasma membrane. However, the individual subunits have different tissue distributions: α1 is ubiquitous; α2 is in skeletal muscle, brain, and heart; α3 is in brain and heart; α4 is present in testis and skeletal muscle; β1 is in most tissues; and β2 is primarily in neural tissues. Different combinations of the α and β subunits can alter the kinetics of enzyme activity. The Na+,K+-ATPase transfers three Na+ ions out of, and two K+ ions into, the cell upon hydrolysis of one ATP molecule. Maintenance of intracellular Na+,K+ levels is necessary for volume regulation, action potentials, and secondary active transport.

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Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
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Citations & References
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Development References (3)

  1. Hernando N, Martin-Vasallo P, Ghosh S, Ghosh PK, Swaroop A, Coca-Prados M. Nucleotide sequence of a cDNA for the beta 2 subunit isoform of Na+,K(+)-ATPase from human retina. Biochim Biophys Acta. 1994; 1189(1):109-111. (Biology). View Reference
  2. Malik N, Canfield VA, Beckers MC, Gros P, Levenson R. Identification of the mammalian Na,K-ATPase 3 subunit. J Biol Chem. 1996; 271(37):22754-22758. (Biology). View Reference
  3. Stengelin MK, Hoffman JF. Na,K-ATPase subunit isoforms in human reticulocytes: evidence from reverse transcription-PCR for the presence of alpha1, alpha3, beta2, beta3, and gamma. Proc Natl Acad Sci U S A. 1997; 94(11):5943-5948. (Biology). View Reference
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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.