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Purified Mouse Anti-Human CD11a
Purified Mouse Anti-Human CD11a

Western blot analysis of CD11a (Integrin αL) on a Jurkat cell lysate (Human T-cell leukemia; ATCC TIB-152).  Lane 1: 1:500, lane 2: 1:1000, lane 3: 1:2000 dilution of the anti-CD11a antibody.

Western blot analysis of CD11a (Integrin αL) on a Jurkat cell lysate (Human T-cell leukemia; ATCC TIB-152).  Lane 1: 1:500, lane 2: 1:1000, lane 3: 1:2000 dilution of the anti-CD11a antibody.

Product Details
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BD Transduction Laboratories™
Integrin αL; LFA-1α
Human (QC Testing)
Mouse IgG1, κ
Human LFA-1α aa. 64-199
Western blot (Routinely Tested), Immunofluorescence (Tested During Development), Immunohistochemistry, Immunoprecipitation (Not Recommended)
180 kDa
250 µg/ml
AB_398146
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.

Recommended Assay Procedures

Western blot:  Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
610826 Rev. 1
Antibody Details
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27/CD11a

CD11a, also known as integrin αL or LFA-1 (lymphocyte function-associated antigen-1), is a member of the β2 family of integrins that includes Mac-1 and p150,95. It is composed of two noncovalently linked α and β chains which are associated with the actin cytoskeleton. CD11a (integrin αL) has been reported to be expressed on the surface of T cells, neutrophils, and  macrophages. It is involved in cell-cell adhesion and it also conveys signals from the plasma membrane to the cytoplasm. Activation of the TCR/CD3 complex on T cells transmits intracellular signals that activate integrin αL ("inside-out" signaling). This results in the conformational change of integrin αL, which increases its affinity for its ligand ICAM-1,-2, and -3. Furthermore, this signaling releases integrin αL from the actin cytoskeleton, resulting in clustering of this surface molecule. The high affinity conformation and the clustering of integrin αL increases the adhesion between it and ICAM. This adhesion is essential for leukocyte activation and results in "outside-in" signaling which induces the polymerization of actin, thereby stabilizing the integrin αL-ICAM interaction. This interaction is necessary for CTL and NK mediated killing, T and B cell responses, ADCC, and immune cell extravasation.

This antibody is routinely tested by western blot analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.

610826 Rev. 1
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
610826 Rev.1
Citations & References
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Development References (4)

  1. Larson RS, Corbi AL, Berman L, Springer T. Primary structure of the leukocyte function-associated molecule-1 alpha subunit: an integrin with an embedded domain defining a protein superfamily. J Cell Biol. 1989; 108(2):703-712. (Biology). View Reference
  2. Lub M, van Kooyk Y, van Vliet SJ, Figdor CG. Dual role of the actin cytoskeleton in regulating cell adhesion mediated by the integrin lymphocyte function-associated molecule-1. Mol Biol Cell. 1997; 8(2):341-351. (Biology). View Reference
  3. Mamane Y, Grandvaux N, Hernandez E, et al. Repression of IRF-4 target genes in human T cell leukemia virus-1 infection. Nature. 2002; 21(44):6751-6765. (Biology: Western blot). View Reference
  4. Nueda A, Lopez-Cabrera M, Vara A, Corbi AL. Characterization of the CD11a (alpha L, LFA-1 alpha) integrin gene promoter. J Biol Chem. 1993; 268(26):19305-19311. (Biology). View Reference
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Please refer to Support Documents for Quality Certificates


Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.