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Purified Mouse Anti-HNF-1α
Product Details
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BD Transduction Laboratories™
Rat (QC Testing), Human, Mouse (Tested in Development)
Mouse IgG1
Mouse HNF-1 aa. 70-269
Western blot (Routinely Tested), Immunofluorescence (Tested During Development)
92 kDa
250 µg/ml
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at -20°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  4. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
610902 Rev. 2
Antibody Details
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2/HNF-1

HNF-1 (Hepatocyte Nuclear Factor 1), a POU-homeodomain containing transcription factor, is a family member of liver-enriched transcription factors that also includes C-EBP, HNF-3, and HNF-4. Although HNF-1 exhibits elevated expression in liver and is thought to be involved in liver organogenesis, it is also found in kidney, stomach, intestine, and at a low level in thymus and spleen. A short N-terminal α-helical segment allows for HNF-1 homodimerization which is followed by DNA binding via an atypical helix-turn-helix motif. The HNF-1 dimer transactivates hepatic genes such as α- and β-fibrinogen, α-fetoprotein, albumin, α-L-antitrypsin, and pyruvate kinase by binding to a highly conserved 13 bp inverted palindromic sequence, GTTAATNATTAAC. HNF-1 represses its own transcription and data suggests that there is coordinate regulation between HNF-1 and HNF-4. Although HNF-4 induces HNF-1 transcription, HNF-1 downregulates HNF-4 activity by binding to the AF2 region in the activation domain of HNF-4. The ability of HNF-1 to dictate the production or activity of itself and other transcription factors indicates its possible function as a general cellular control mechanism.

610902 Rev. 2
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
610902 Rev.2
Citations & References
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Development References (5)

  1. Frain M, Swart G, Monaci P, et al. The liver-specific transcription factor LF-B1 contains a highly diverged homeobox DNA binding domain. Cell. 1989; 59(1):145-157. (Biology). View Reference
  2. Kritis AA, Ktistaki E, Barda D, Zannis VI, Talianidis I. An indirect negative autoregulatory mechanism involved in hepatocyte nuclear factor-1 gene expression. Nucleic Acids Res. 1993; 21(25):5882-5889. (Biology). View Reference
  3. Ktistaki E, Talianidis I. Modulation of hepatic gene expression by hepatocyte nuclear factor 1. Science. 1997; 277(5322):109-112. (Biology). View Reference
  4. Kuo CJ, Conley PB, Hsieh CL, Francke U, Crabtree GR. Molecular cloning, functional expression, and chromosomal localization of mouse hepatocyte nuclear factor 1. Proc Natl Acad Sci U S A. 1990; 87(24):9838-9842. (Biology). View Reference
  5. Tian JM, Schibler U. Tissue-specific expression of the gene encoding hepatocyte nuclear factor 1 may involve hepatocyte nuclear factor 4. Genes Dev. 1991; 5(12A):2225-2234. (Biology). View Reference
View All (5) View Less
610902 Rev. 2

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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.