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Purified Mouse Anti-HDAC3
Purified Mouse Anti-HDAC3

Western blot analysis of HDAC3 on a human endothelial cell lysate. Lane 1: 1:500, lane 2: 1:1000, lane 3: 1:2000 dilution of the mouse anti-HDAC3 antibody.

Purified Mouse Anti-HDAC3

Immunofluorescence staining of human endothelial cells.

Western blot analysis of HDAC3 on a human endothelial cell lysate. Lane 1: 1:500, lane 2: 1:1000, lane 3: 1:2000 dilution of the mouse anti-HDAC3 antibody.

Immunofluorescence staining of human endothelial cells.

Product Details
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BD Transduction Laboratories™
Histone Deacetylase-3
Human (QC Testing), Mouse, Rat, Dog (Tested in Development)
Mouse IgG1
Human HDAC3 aa. 309-425
Western blot (Routinely Tested), Immunofluorescence (Tested During Development)
50 kDa
250 µg/ml
AB_398436
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.

Recommended Assay Procedures

Western blot:  Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
611125 Rev. 1
Antibody Details
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40/HDAC3

Regulation of gene expression occurs at multiple levels and is mediated by numerous factors.  DNA-binding proteins such as histones function to spatially organize the DNA into chromatin.  The spatial organization of chromatin is also important for gene expression, and modifications to DNA-binding proteins have profound effects on gene expression.  Human Histone Deacetylase 3 (HDAC3) is a protein of approximately 50 kDa that removes acetyl groups from the ε-amino groups of lysines in histones, thus in competition with the acetylases.  HDAC3 is the third member of this family of deacetylases, related to the yeast RPD3 gene, which affect transcription of certain human genes.  HDAC3 importance is reflected in its distribution in virtually every cell type examined.  In in vitro assays, HDAC3 exhibited activity towards histones H3 and H4 acetylated in vivo.  Furthermore, inhibitors of deacetylation, such as sodium butyrate, inhibited HDAC3 activity.  Further studies will determine the specific role of HDAC3 in regulating gene expression.

611125 Rev. 1
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
611125 Rev.1
Citations & References
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Development References (1)

  1. Emiliani S, Fischle W, Van Lint C, Al-Abed Y, Verdin E. Characterization of a human RPD3 ortholog, HDAC3. Proc Natl Acad Sci U S A. 1998; 95(6):2795-2800. (Biology). View Reference
611125 Rev. 1

Please refer to Support Documents for Quality Certificates


Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.